FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001839766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5665584
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC59145610.439453373209187No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1359652.3998408637132553No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT849401.499227617135321Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC311340.5495285216846136TruSeq Adapter, Index 3 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC301010.5312956263643783No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT253240.44697951702772387No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT225860.3986526366919986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC182820.32268518126286716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT182670.3224204247964552Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT174760.30845893380099915No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT139890.2469118805757712Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC124960.22055978695223652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG102760.18137582992327003TruSeq Adapter, Index 3 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC99690.17595714757737244No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA94910.16752024151437875Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG94710.16716723289249616TruSeq Adapter, Index 3 (100% over 37bp)
CAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC93550.16511978288557722Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT86270.15227026904905125Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC83510.14739875006707165No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT83480.14734579877378923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCTGAGGGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT81750.14429227419450494TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)