FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22011074
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA18611628.455571045738159Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11147425.06445982599486No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3591511.6316832154578191Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3056591.3886600899165575No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2841701.2910319596399522Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAGTCTGACATGTGTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1556870.7073121466040231Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1443240.6556881322556092TruSeq Adapter, Index 7 (100% over 50bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1266220.5752649779833552TruSeq Adapter, Index 7 (100% over 36bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1217120.5529580246743071Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1068390.48538749176891594Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT810540.36824191313881366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA786330.35724290418541144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA756680.3437724120140616Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC754970.342995530340773Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGATGTAGCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC738480.3355038468363697Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC666310.3027158056894452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA606230.2754204542677018TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC549520.24965615035413535No Hit
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA546760.24840223607444142Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC544290.24728007365746896Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCGGGACGTGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC524610.23833911966312957Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGTCTGACATGTGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA515410.2341594053974831Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT472750.21477825207438764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT465420.21144811016491064Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC455560.20696854683238086No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC431710.19613309191546038Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCAGAATCCGGGCTAGAAGCGACGAGATCGGAAGAGCACACGTCTGAAC426090.193579831679272Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT418520.19014065374547376Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT412400.18736023512528285Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT405070.18403009321580582No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA395760.17980040410567882Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT343300.15596694645613385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG332130.15089222815751743Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT304220.13821224716249647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC302350.13736267480632702No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA282190.12820364876334522TruSeq Adapter, Index 7 (100% over 37bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA257780.11711377645634194TruSeq Adapter, Index 7 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT249640.11341563796478082No Hit
ACCGGGACGTGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC239120.10863622556536769Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAAATAGGAAGATCGGAAGAGCACACGTCTGAACTCCA232670.1057058824117351Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)