FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13456303
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA603541844.85197754539267Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4093933.0423883885492176No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA2403091.7858471230916841Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT897470.6669513907348846Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG892220.6630498733567459Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT766400.5695472225915246No Hit
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC431860.3209351037948536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC326100.24233996514495845TruSeq Adapter, Index 12 (100% over 50bp)
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA292910.2176749438534492Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA233560.1735692188263002Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT232850.1730415850475424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA221960.16494872328603183Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA217500.1616342913800321Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC207140.1539352970871717No Hit
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA204910.15227808113417185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA183840.13661999138990852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA174110.1293891791824248Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA168710.1253761898791964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT168620.1253093067241426Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC148830.11060244407397782Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC146630.1089675225059959No Hit
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG141240.10496196466444016Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT138590.10299262732118919No Hit
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG136210.10122393944309964Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)