FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001841278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405064510
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACGTT43675931.0782462773645611No Hit
CTCACGCC39099700.9652709391894146No Hit
GACCACTT38520760.9509784009465554ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GGCCGCTC37146570.9170531874046434No Hit
CCGACCGC36187490.8933759711508668No Hit
GCAGCTGG35590790.8786449842273272No Hit
GAGGTGCG35223560.8695790208823775No Hit
GAAGCGAT33815490.8348173973572752No Hit
GTCCTCAA33623910.8300877803389884No Hit
TACTCCGG33539950.8280150240760417No Hit
CTCCTTCA33511430.8273109386946786No Hit
TGCTTCTT33388840.8242845071764001No Hit
GTTCGGAC33292670.8219103174454854No Hit
CCGAAGGT33273620.8214400219856338No Hit
CGTGTGGT32772620.8090716217029232No Hit
CAGCGTCT32457210.8012849607584728No Hit
TCCGGTCT32395790.7997686590711194No Hit
TATAATCC32258510.7963795692691024No Hit
TCGTCGAC32219620.7954194752830851No Hit
TCAGGTAC32101230.7924967309528056No Hit
GTTGGTCG31913280.7878567292898606No Hit
GTGGCGAA31642840.7811802618797682No Hit
GCTGGAAT31507170.7778309188331508No Hit
GTTGCAAC31105670.7679189174089827No Hit
GCCGTCCT30898400.7628019546812432No Hit
GTGCGGTG30883200.7624267058103905No Hit
CGTGATCC30853390.7616907736498565No Hit
GCACCACT30450760.7517508754346314No Hit
CTAGTCCG30410100.7507470847051004No Hit
CACTGCTT29964730.7397520459148593Clontech Universal Primer Mix Long (100% over 8bp)
ACAGCGGA29933430.7389793294900113No Hit
CTGACTGG29930940.7389178577999835No Hit
TCTAGTGA29639000.7317106107370404No Hit
ACTCTTGT29235520.7217497282099585No Hit
CAGAGTGC29098110.7183574290425987No Hit
GGATACTA29035150.7168031087196457No Hit
CTATGGAG28871260.7127570865193793No Hit
GGCTGAGT28851190.7122616098852995No Hit
ATTGACCT28716350.7089327574020247No Hit
GCATCCGT28712160.708829317088283No Hit
TGGCTGTG28557850.7050198004263567No Hit
GTGGTCGT28231690.6969677496554808No Hit
CGCAAGTG27007470.6667449093478962No Hit
AACTACCT26999830.6665562974154413No Hit
ATTATCGT26825300.6622476010055781No Hit
GTACTATA26716740.6595675340700671No Hit
TCCTGGAA26292500.6490941405851626No Hit
TGCATCAA26142190.6453833736260923No Hit
CCAATCTA26067050.6435283604579428No Hit
CAATTAAG26057330.6432883986800029No Hit
AGCCATTG25900710.6394218540646772No Hit
TGCCAGGT25807850.6371293797128759No Hit
TGCAGTGT25757650.6358900709420334No Hit
GGACACAT25493800.6293762936673963No Hit
TTAGAGAT25478760.6290049947846579No Hit
ATTAATCA25314490.6249495913626202No Hit
CCTACTCG25070160.6189177126379203No Hit
GATTATCT25018430.6176406321057355No Hit
TTACAATT24868340.6139352963803222No Hit
CATACGTT24867820.6139224589189509No Hit
ATATTACA24750500.6110261301341853No Hit
TCGAGCAA24727320.610453875606135No Hit
ATGCAACT24652170.6085986155637283No Hit
TATCAAGA24632310.6081083232890485No Hit
GGTTCAAT24467500.6040395886571253No Hit
GTTACCAA24061180.5940085938410157No Hit
ACCGTTCA23954040.5913635830500183No Hit
GCCGGTTG23847080.5887230159956497No Hit
GCTTCTCA23292940.5750427259104975No Hit
CATCGAGC23255620.5741213911828513No Hit
AGGATTGG22853610.564196799171569No Hit
AGATGGAC22777790.562324998553934RNA PCR Primer, Index 16 (100% over 8bp)
GCTCCAGA22728180.5611002553642629No Hit
CGACTCTG22597720.5578795338056154No Hit
TTATCGTC21872420.5399737439352561No Hit
GCAGATCT21841280.5392049774985224No Hit
TACACACA21672310.535033543175629No Hit
TCGTCTGG21656820.5346511349513192No Hit
CCGACACA21492610.5305972127748244No Hit
AATCTGTA21432600.5291157203577277No Hit
TGATTCGC21206740.5235398183859653No Hit
GCGACAAG21189400.5231117384240846No Hit
AGCATAGT21182070.522930779593601No Hit
GAACAGAC21160840.5224066655456929No Hit
GTCCATCC20950260.5172079874388403No Hit
GCGTACGA20918630.5164271241635067No Hit
GAGCTTAG20659530.5100306121610111No Hit
AGACAGAG20608670.5087750096891974No Hit
TCCGCGAT20297640.5010964796693741No Hit
CGTTCATA20027360.4944239622473961No Hit
TTGACCGA19844880.4899190008031067No Hit
GAGTCCTA19697770.4862872336063211No Hit
CGGACATC19610110.48412313386823247No Hit
AACGGATA19525270.4820286526706573No Hit
AGCTCACG19471920.48071157850881574No Hit
TCCGTATG19394060.4787894155427243No Hit
TAGGTCTC19314050.47681417461134773No Hit
ACGCGACC18610970.4594569393403534No Hit
ACAGCACG18582130.4587449539827619No Hit
ATCCTAAC18545150.4578320129798584No Hit
ACATACTC18285410.45141970102490586No Hit
GTATCTAG17876510.4413250126504541No Hit
AAGTGAGA17821390.43996424174509885No Hit
CTACAAGC17426620.4302183866959857No Hit
ACCGAACT16793950.41459939306951377No Hit
ATCATCTC16581710.40935973383597585Illumina PCR Primer Index 7 (100% over 8bp)
TATAGGTG16016570.39540788206796984RNA PCR Primer, Index 39 (100% over 8bp)
AGAACGGT15736680.38849811848488036No Hit
GAAGGTGA15531360.3834292962372833No Hit
GTAATTCC15498240.38261164869763586No Hit
ATGTCAAC15469220.38189521960341577No Hit
ACCTGAAC15036660.37121642673657096No Hit
GCGAGATA14168150.34977515062971076No Hit
GGCAACAC14011100.3458979904213282No Hit
AGTCATGA13852530.3419833053258603No Hit
GGAATATC12251950.3024691054765573RNA PCR Primer, Index 37 (100% over 8bp)
AGGAATAC12014440.29660559499522676No Hit
GTCTGGCG11888480.29349596685229223No Hit
AGAGCCGC11805270.2914417261586309No Hit
CCGTACAC11209090.27672357669646247No Hit
TAGCGCTG10904200.2691966274705232No Hit
AGCGTGCG10885050.2687238632681002No Hit
AGGCCAGA10494970.25909379224558576No Hit
TGCTAACT9851010.24319607758280282No Hit
ACACTGTG9742880.24052662624034876No Hit
AACGTCGA5317960.13128674244998656No Hit
GCTGGGAT4592540.1133779900885417ABI Dynabead EcoP Oligo (100% over 8bp)
GTTCGGGC4230520.10444064823156195No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)