FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001841286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416690129
Sequences flagged as poor quality0
Sequence length6
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAT136903073.2854886754468815No Hit
CTTGTA110351882.648295995511811No Hit
TTCCAT109299342.623036457866272No Hit
CTATTA109067802.6174798107588484No Hit
GACTAA107368642.57670226692603No Hit
GTACTT105858862.540469587173734No Hit
GCCAAT105028282.5205367895815933No Hit
TTCTGT101767272.442276956361498No Hit
AAGCAT101156562.427620741647086No Hit
CAGTCC100687672.4163680152836067No Hit
CAGATC100548242.4130218837029376No Hit
GCGTAT98907822.3736540204915677No Hit
AACATA95285852.2867316350563227No Hit
TGCGGA94610462.270523187747507No Hit
TGATAA92869222.2287357807796786No Hit
TGTGAT92590412.222044717550773No Hit
ATACCT92390732.2172526673892No Hit
GCACAT91465272.195042878013556No Hit
ATCAGC91043802.1849281675688554No Hit
CGTACT90956802.18284028513668No Hit
AGTTCC90678732.1761669809077717No Hit
TCGGAC90536202.172746453516301No Hit
GTCTCA89843242.1561163499507807No Hit
TTACGT87544992.100961455700814No Hit
ATGTGA87278792.0945730154313305No Hit
ACCGCT85903922.0615779933678247No Hit
AGCCTA81515841.9562700032186267No Hit
TAAGTC79682621.9122752005483672No Hit
ACAGTG78883711.8931024401588357No Hit
ACAAGT78121191.8748029905935208No Hit
GGTGGT75988001.8236093132890123No Hit
GTAAGA75720691.817194234519532No Hit
AACCTT75248001.8058503132911987No Hit
CGTTGC73966311.7750914853084987No Hit
AGTCAA71766921.7223090974636455No Hit
GACCGA70791081.6988902561692312No Hit
ACTAGC70528011.6925769316700083No Hit
CAGGAC70452141.6907561541972598No Hit
TATTGG69810101.6753480618207781No Hit
TATTCT67799051.6270855794618548No Hit
CGTCAG66178251.5881885697369136No Hit
AGACGG63694381.528579046325333No Hit
ATGCCG61180461.4682483635219494No Hit
GGTACC60277581.446580463632725No Hit
GAGCCA59366551.4247169747570383No Hit
CTGGAG50772621.2184742682013474No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)