FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001841306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400066378
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGTT92960122.323617407309344No Hit
TAGTCGTG92869742.3213582821998604No Hit
GAGTAATT90884702.2717405160200688No Hit
TAGCTGGT89951482.248413886957529No Hit
GAGACCAT88428292.210340455053186No Hit
GTTGTGTG88194172.2044884261681195No Hit
GGATCTCT87200632.1796540473091195No Hit
ACGTGCGC86572062.1639424045776723No Hit
TCTCTATA86078072.151594703616908No Hit
CATCGAGC86039182.1506226149301653No Hit
CAACCGGT85779212.1441244432692614No Hit
ACTTCTAT84825632.1202888986587123No Hit
GTCAACGT83656232.0910587492558546No Hit
TAATTGAT83526582.087818037035844No Hit
TGTAGACG83343572.083243546149734No Hit
ATTATCGT81221632.0302038478224733No Hit
GATTGGAT81119952.027662269584674No Hit
GTTCGGAC80867022.0213400687222958No Hit
AGCACAAC79850521.9959317850999216No Hit
ACATTCCT79732871.9929910231046708TruSeq Adapter, Index 27 (100% over 8bp)
CTGTTCGC79432421.9854810193522434No Hit
AGTTCCGG79200951.9796952294751449No Hit
CAGAGGAT78498761.9621433921147953No Hit
TTGTACTC78430311.9604324260410606No Hit
GTCAGATG77909511.947414586286479No Hit
AAGTTACG76573111.9140101295890455No Hit
ACAGCACG76455331.9110661181330264No Hit
CCAACAAT76045911.9008323163812582RNA PCR Primer, Index 36 (100% over 8bp)
GAAGCGAT75701811.8922312436862665No Hit
CGCGTGAA75384851.8843085584162735No Hit
TTCGGAAG75367311.8838701311710826No Hit
CGACAGTT74519731.862684146879246No Hit
GATTATCT72267211.8063804901895555No Hit
TGCTGCCG71601481.7897400015954352No Hit
GCCTGTGA70180591.7542236453571711No Hit
GCGCTGGC69602371.7397705437771127No Hit
CAACGTAA68543031.7132914378523454No Hit
TTCACTTG68116131.7026207086065104No Hit
GGCACGTT67746131.6933722433430782No Hit
AGCGCTTC67449641.6859612231648218No Hit
CATCCTCA66422001.660274485750462No Hit
TCCTGGAA66405841.6598705527811188No Hit
AGGCCGAC65347471.6334156928328527No Hit
CACTCTCG59972811.4990714865821593No Hit
ACATCCAG58831181.4705354719911004No Hit
ATCATGGA47310251.1825600100791276No Hit
GGGTCTCT5733170.14330546917391795No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)