FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001841312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385223709
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGAGT105348132.734726018641807No Hit
TAGCTGGT98771212.5639961324395015No Hit
GTGGCGAA97437762.529381180949068No Hit
ACCAGCGG96984712.517620482180654No Hit
TAGTCGTG96856412.514289949895062No Hit
CTCTATAG96851142.514153146269613No Hit
CGACAGTT96249562.4985367658146918No Hit
GCCGTCCT95274202.473217451940374No Hit
TAATGATT93262662.421000001326502No Hit
CGGCCAAG92569652.403010194785285No Hit
GAGAGCGG91790872.3827938897706837No Hit
TGTACCTA90087522.33857672555663No Hit
TGCCAGGT89079142.3124002474105247No Hit
ACTAAGTG88477262.29677607927294No Hit
TCGCGGAT88429402.2955336842987513No Hit
AGGCCGAC88366312.293895934634698No Hit
GGCACGTT87614052.2743680607675163No Hit
GAGGTGCG87093092.2608444902335956No Hit
TTGTCACA85616842.2225226017955193No Hit
GACGCTCA85486352.2191352194264864No Hit
GGATCTCT84974582.2058502115714793No Hit
ACATTCCT84596652.19603954854191TruSeq Adapter, Index 27 (100% over 8bp)
CATAATAA83180282.1592720815633912No Hit
TGTAGACG82150192.1325320347818986No Hit
ATGTTGTG80588782.091999482825186No Hit
AGCGCTTC79989192.076434760665263No Hit
GTCCAGAG79581322.0658468869059146No Hit
CTCTCTGC78493732.0376142009473255No Hit
CATCCTCA77383382.008790689464028No Hit
ACCACCAA76895881.9961357051364665No Hit
CTGCTCAG76661281.990045737293911No Hit
GATGTCCA76625131.9891073215330057No Hit
GATATATG76215491.9784735004459448No Hit
GGAGAACC75756291.9665531541829373No Hit
ATTATCGT75586741.962151815531167No Hit
AACCTTCT74865941.9434406099859236No Hit
TGCCTGAC74463011.9329809734010945No Hit
GGTGCGGA73721771.913739167077071No Hit
AAGTTACG71417071.8539115929648038No Hit
GGTTGCAG69069861.7929805042191729No Hit
CCGGTCTT5967340.15490583420970072No Hit
CTATGGAG5798750.15052941614245244No Hit
GGTCAACT5728220.1486985319483542No Hit
AACTACCT5114590.13276934623979753No Hit
CTTAGATT4246730.11024061865309541No Hit
AACTAGTC4171040.1082757863171916No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)