FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001841314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407113415
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAAC114132392.803454413311337No Hit
AGCATAGT102437602.5161931841523817No Hit
GAAGCGAT101323592.4888295562552267No Hit
AACGCTGG100431712.466922147480697No Hit
GTCAGATG99854872.452753122861353No Hit
TGCCTAGA99460212.4430590183327663No Hit
CAGCGTCT97847532.4034464695789994No Hit
CGCTGAAG97506462.3950687058543623No Hit
TAAGCAAC96346022.3665646095204207No Hit
TTCGGAAG95405802.34346981663574No Hit
GGCCTGCT94633372.3244964796849055No Hit
AGGTGTAA94002692.309004973466669No Hit
GCAACAGC93935422.3073526083634457No Hit
CGTTCGCG93136822.287736452997993No Hit
AACGAGAT92817042.27988163937069No Hit
TCAGGTAC92319522.267660966170815No Hit
TGAGGCGG91429642.2458026837558274No Hit
AGTTCGTC91162282.2392354720121417No Hit
TCGAGCAA90206352.2157547915732523No Hit
CACGGCAA89364692.1950809456868425No Hit
ATATTACA88983702.185722619825731No Hit
AAGACACT88666772.1779378112607763No Hit
AACTCTAC88518132.174286740219553No Hit
GATTGGAT88327822.169612121477255No Hit
CGCATATA87142392.1404941912808253No Hit
CTCCTTCA86289442.1195430270947964No Hit
ACTTCTAT85101012.0903514073590523No Hit
CGCTACAA83744012.057019172409241No Hit
GGAAGGCG83151042.042453943700185No Hit
CTTCCTGT82323342.022122999803384No Hit
ATAACGCG81305561.9971230866956327No Hit
GATTATCT81170021.9937937933094148No Hit
CCTTAGCC79512791.9530869548968315No Hit
TGCATCAA78508951.9284294525150933No Hit
AGACAGAG77511191.9039212942663655No Hit
TATAATCC75192251.8469607541672386No Hit
CCAATCTA74974251.84160598097707No Hit
AGGCGTGC73058551.7945502974889689No Hit
CGAAGCAA72074411.7703766897487274No Hit
GAGTGGCA70384801.7288744955751458No Hit
AGGATTGG4229110.1038803892030922No Hit
TCGTCTGG4182970.1027470440884391No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)