FastQCFastQC Report
Tue 8 Aug 2023
EGAF00001841320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414283773
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATAGC105833642.554617074031524No Hit
TGCCAGGT105096442.5368225078900206No Hit
ATAACGCG101464932.4491649592078035No Hit
GAGCCGTC99952722.4126631674757872No Hit
TAGCTGGT99190622.394267564035147No Hit
GAGAGCGG93373652.2538572854022934No Hit
ATTATCGT92462522.2318643892431673No Hit
TGCTGCCG91460082.2076674482734324No Hit
GAAGCGAT91431622.2069804795371506No Hit
ACCGTTCA88114792.126918690585547No Hit
TTCGGAAG87167672.104057066217749No Hit
GATGTCCA86714672.09312253222141No Hit
TTGAGAGT86633582.0911651782219334No Hit
CGACAGTT83521092.016035757210312No Hit
CGTGCCAC83041392.004456737435381No Hit
CATCGAGC82252851.985422924107626No Hit
GGCACGTT81941161.9778993371289972No Hit
ACTAAGTG81774111.9738670768550717No Hit
AGCACAAC81423421.9654021061549036No Hit
GGATCTCT80921491.9532864976586954No Hit
AACTACCT80535551.943970660902521No Hit
AGACAGAG79905371.9287593482450978No Hit
CCTTAGCC79724361.924390120874949No Hit
GTCAGATG79584651.9210177947278664No Hit
TGTAGACG79273181.913499518118949No Hit
TGCCTAGA78997581.906847073153406No Hit
CAGCGTCT78993351.9067449692266851No Hit
GGTGCGGA78217251.8880114331680569No Hit
CGTTCGCG77460701.8697497958724056No Hit
ATGTTGTG77360901.867340819067031No Hit
TAAGCAAC77337791.866782988866909No Hit
TATTAGAG76989221.858369190820322No Hit
CTCACGCC76391901.843951054293406No Hit
GATTATCT74686501.8027860338135908No Hit
GTTCGGAC74597191.8006302650912662No Hit
CCAACAAT74321331.7939715442342463RNA PCR Primer, Index 36 (100% over 8bp)
GGTTGCAG73991321.7860057482869358No Hit
GCCTGTGA73965511.78538274536763No Hit
CTAACATA72805351.7573787520758146No Hit
CGGCCAAG72143781.741409746212773No Hit
GGAAGGCG71942451.736550033785658No Hit
CGCGCGCT71236631.7195129194693322No Hit
GTGGCGAA71200481.7186403291735974No Hit
CTCTATAG69923851.68782497788056No Hit
CATAATAA67058631.618664171043938No Hit
CTATGGAG61892501.4939638970604818No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)