FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001841324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415005544
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAAC141639753.4129604302346377No Hit
ATCCTAAC133161633.2086711111502644No Hit
GTCAACGT114295662.7540754973625123No Hit
TCCGATGC108349622.6107993391047324No Hit
ATGCTAGG107628922.593433306037955No Hit
GCCACGAC100957962.4326894293248285No Hit
ACATTCCT100473222.421009103435013TruSeq Adapter, Index 27 (100% over 8bp)
TCGGTAAT99103192.3879967733635867No Hit
CTTAGATT97041622.3383210514411825No Hit
TCAGGTAC94799322.284290447936763No Hit
ACGAGTTC93570192.254673253232492No Hit
TGCCTGAC90569562.1823698817864465No Hit
TCTCTATA90062502.1701517317561425No Hit
ACGCGACC89956652.1676011634196386No Hit
CTCCTTCA88397772.130038291729423No Hit
TGTACGCC86672372.0884629435215447No Hit
CTTCCTGT86111172.074940232605664No Hit
TGAGGCGG82263161.9822183387506747No Hit
GCAACAGC82134191.9791106694227683No Hit
CTGGCCTC81869471.9727319594554622No Hit
TAACATTA81788831.9707888528833726No Hit
AACGAGAT80727011.9452031705870416No Hit
CGCTACAA80672531.9438904170398266No Hit
ACTCGGAA77993161.879328146999405No Hit
AGTTCCGG77669991.871541022112225No Hit
TTGTCACA76407791.8411269705833135No Hit
GGCCAATA76269091.8377848465561701No Hit
CAATTAAG76212901.8364308887401273No Hit
CATTAAGT75353031.815711406496295No Hit
GTCCTCAA73580671.773004507139789No Hit
GCCTCATT73337681.7671494046354232No Hit
AGGAATAC72717791.7522124957443943No Hit
TGATAAGG72589921.7491313320864936No Hit
AAGTTACG72288141.7418596220006162No Hit
TACGTCAG69536481.6755554475195156No Hit
CGAAGCAA67815211.6340796160544786No Hit
TAATGATT67414531.6244248052744086No Hit
CATCCTCA67413831.6244079380298593No Hit
ACCACCAA66289851.5973244444175427No Hit
CGCATATA61017691.4702861415268225No Hit
CACGGCAA59546101.434826615231916No Hit
CCAGATTC59201871.426532027244436No Hit
AGGCCGAC59198931.4264611848173288No Hit
AGCTGTGG57871921.39448546740378No Hit
ATCATGGA55128621.3283827360147267No Hit
GGTCAACT48631711.1718327791784873No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)