FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001841328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367035375
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACGT98929362.695363083190551No Hit
CAGAGGAT98778782.691260481363683No Hit
GAGCCGTC97365192.6527467549960275No Hit
GCCTCATT89491562.4382271054935782No Hit
AGCGCTTC88172292.4022831586737383No Hit
TCGCGGAT86405732.354152648092844No Hit
CTGTTCGC83698722.2803992666919366No Hit
GGCCAATA82944992.2598636439335036No Hit
GTGGCGAA81655292.2247253415287287No Hit
CTAACATA81204232.2124360628726865No Hit
TTCACTTG79018162.152875863804681No Hit
GAGACCAT76017022.0711088134215947No Hit
ATAGGAGG75701172.0625033758666995No Hit
CTCTTAAT75529702.0578316190912114No Hit
CGTGCCAC74771822.037182928212301No Hit
GTCAGATG74209962.021874866966161No Hit
TGTAGACG72577201.977389781570782No Hit
TGCCAGGT72497391.9752153317646834No Hit
ACCACCAA72290521.9695790902988575No Hit
CAGATTCA71927451.9596871282502402No Hit
GTCAACGT71705161.9536307637921821No Hit
CGACAGTT71477071.947416376418758No Hit
AGCTGTGG70176061.911969929329019No Hit
AACTACCT68832711.8753699149571073No Hit
CAGCGTCT68607871.8692440749069488No Hit
GCTCCAGA67895231.849827962767894No Hit
TTCGGAAG67514681.8394597523467595No Hit
GGATCAGA67255081.8323868646176134No Hit
GCCACGAC67136141.8291463050393986No Hit
ATATTACA67044971.8266623482818243No Hit
CAATTAAG66664141.8162865091682239No Hit
ATCATGGA66147441.8022088470355209No Hit
GAGTAATT65499781.7845631364551713No Hit
TGCATCAA65486591.7842037705493647No Hit
CGCGTGAA65433241.7827502321812985No Hit
TTGGAACG65214121.7767802354200872No Hit
TAGTCGTG64907321.7684213681038237No Hit
ACGCGACC64360261.7535165377451696No Hit
CTGCTCAG62961861.7154166679437914No Hit
AGCCATTG62755311.7097891449836409No Hit
TTGTCACA61785061.683354363322609No Hit
TACGTCAG61075531.6640229841605867No Hit
TGATAAGG60421111.6461930951478452No Hit
CTATGGAG59610471.6241069406457076No Hit
AGACAGAG58576021.5959230087835539No Hit
CGCGCGCT36709791.0001703514272977No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)