FastQCFastQC Report
Tue 19 Sep 2023
EGAF00001841334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405295760
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGGAT100378922.476683200436146No Hit
TTGAGAGT98661352.4343050122211984No Hit
GTATGCCT98485742.4299721270215113No Hit
AGGTGTAA95007232.3441456678451313No Hit
TCTACCGT89909422.2183656695545No Hit
AGGAATAC89709362.213429521197064No Hit
TATTAGAG88307342.1788370053513515No Hit
AGAGTATT86625662.137344343301297No Hit
CGACAGTT84936772.095673786471391No Hit
AACGCTGG84926222.095413482736656No Hit
AGCATAGT84489262.0846322201840946No Hit
TATATAGC82330712.0313735825906494No Hit
ATCTGTCT81822292.0188291631770343No Hit
CCAACAAT80958521.997517072470731RNA PCR Primer, Index 36 (100% over 8bp)
ACTCGGAA80469151.9854426801800247No Hit
CAACGTAA80372711.9830631832911352No Hit
GCCGTCCT80323711.9818541896416582No Hit
GTTCGGAC80006911.974037675597692No Hit
GTTGTGTG78409031.9346126394216412No Hit
TCGAGCAA78383091.9339726129876118No Hit
ATGCAACT77561871.913710372889171No Hit
TCGGCCAG76070151.876904658464722No Hit
CAATATGG75381011.859901273085117No Hit
AACTACCT75240461.8564334351783003No Hit
CCTTACTG73935841.8242441026276712No Hit
CTAACATA73695641.818317566411255No Hit
ACCTGAAC73691751.8182215871194902No Hit
ATAGGAGG73499971.8134897340154756No Hit
CTATGGAG72917881.7991276296598808No Hit
GCGCTGGC72738581.794703699836386No Hit
CTGCTCAG72631131.7920525494764614No Hit
ACAGCACG72462461.7878908972548835No Hit
GTTGGTCG72424771.7869609590783777No Hit
CGTGCCAC71726161.7697239171710062No Hit
CAGATTCA70706791.7445726547941187No Hit
GAGACCAT69659821.7187404082391586No Hit
ATATGGTT69374521.7117011043984276No Hit
CATCGAGC69053101.7037705995246535No Hit
CGAACGAG68970181.7017246861896607No Hit
GGTTGCAG68882561.6995628081576772No Hit
GCTCACGT68630791.6933508013999454No Hit
TGCTGCCG68274411.6845577165672792No Hit
TGTAGACG66388471.63802527813269No Hit
TGTACGCC66243321.6344439428628614No Hit
TCCTGGAA65825701.6241398627017467No Hit
GGATCAGA64006901.5792639923003389No Hit
TGGCTTCC57994561.4309194845759057No Hit
CTGTTCGC55165501.3611171259230543No Hit
GAGGTGCG51026611.2589968866192924No Hit
CGAACTGC48176571.1886768812977466No Hit
GTCATTAG41574021.0257699216986627No Hit
CAAGCACT39639620.9780418132180805No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)