FastQCFastQC Report
Wed 20 Sep 2023
EGAF00001841340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413867205
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATAA116144772.806329387707828No Hit
TCGGTAAT103110112.491381504847672No Hit
AGCTGTGG102087832.466680828213968No Hit
ACATTCCT101286962.447329935214364TruSeq Adapter, Index 27 (100% over 8bp)
GCCTGTGA100205742.4212051302784428No Hit
TCCGATGC98567342.381617552905648No Hit
TTCGTTCG98295682.3750536117013668No Hit
ATTGAGTA96547072.332803102869675No Hit
TAATGATT95906662.317329298899148No Hit
CTCTTAAT94973022.294770372056902No Hit
TAATTGAT94733892.288992431763227No Hit
CTCACGCC93578512.261075747714777No Hit
GGTGCGGA93398102.256716620008585No Hit
TGAGGCGG90842272.194961787320162No Hit
AGCGCTTC90741582.192528881335258No Hit
TATCTACT90566922.18830868708237No Hit
GCAACAGC89623122.1655042708687198No Hit
GGAAGGCG89238012.1561991122248982No Hit
AGCACAAC88446242.137068096516611No Hit
TAAGCAAC88299002.1335104336184356No Hit
TCTCTATA88183122.130710501693412No Hit
CGTTCGCG87266962.1085739325492097No Hit
GAGCCGTC85546472.067002868710025No Hit
TATGGTGG85523102.0664381948311177No Hit
TGATAAGG84386742.0389810784838582No Hit
ACGAGTTC84314722.0372409067782984No Hit
TTGGAACG82976552.004907588655158No Hit
ACGTGCGC82961372.0045408043384354No Hit
CACTCTCG82070311.9830107099208307No Hit
GGTCAACT80959671.956175048950786No Hit
TAGTCGTG80487711.9447713911035787No Hit
CGCGCGCT80432981.94344898625152No Hit
TAACATTA77095551.8628088688496107No Hit
ACCGTTCA76778841.8551564142416166No Hit
GCCACGAC76561191.8498974810048068No Hit
TTGTCACA75408711.8220508677415017No Hit
ATGCTAGG75369651.8211070867526218No Hit
AGGCCGAC75067521.813806919057527No Hit
CTTAGATT73676241.7801903390726501No Hit
CCTTAGCC72735661.7574637255928505No Hit
ACCACCAA72392951.749183050152524No Hit
AGTTCCGG71632681.730813148144947No Hit
GCCTCATT68749191.6611412832287593No Hit
CCAGATTC67091461.6210866478294652No Hit
TGCCTGAC65632881.5858439423824362No Hit
ACATCCAG58827311.4214054481557679No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)