FastQCFastQC Report
Wed 13 Sep 2023
EGAF00001841344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411592943
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGCT136610993.319079987238751No Hit
GTCAACGT126457423.072390383525113No Hit
CTCACCTT122431812.9745847707597846No Hit
GATGTCCA116668752.8345663351181414No Hit
TAGAGTAG111686632.713521499808611No Hit
AGCGCTTC109384072.657578849693737No Hit
GGATCAGA108648532.639708280907042No Hit
GCAACAGC108599042.638505879339141No Hit
CTCACGCC107883862.621129974038452No Hit
GCTCCAGA106955342.5985707923082635No Hit
AGGATTGG106014282.5757069406313895No Hit
TGAGGCGG104717042.5441893934512865No Hit
GGAAGGCG104165472.5307885320084313No Hit
TAAGCAAC103196152.507238079638309No Hit
TAGTCGTG100519612.442209267907686No Hit
AAGTTACG100443732.4403656988842006No Hit
ACCGTTCA96771232.3511391933656163No Hit
ATGTTGTG96000542.332414625485938No Hit
GTCCAGAG95287672.3150948435964804No Hit
AATTGCTA94687732.3005187919366246No Hit
ATCATGGA92772082.253976448765304No Hit
CATCCTCA91876362.2322141708829055No Hit
CGGCCAAG91785312.230002033829817No Hit
CGTCCGAA88950462.1611269462411555No Hit
CTACGGCC88250612.1441234963059124No Hit
TCGTCTGG87644112.129388063876499No Hit
GATATATG87451582.124710384065064No Hit
GAGAGCGG86686542.1061230877323376No Hit
CTGTAGCA85197552.069946811503034No Hit
CCAACAAT83871862.037738047418369RNA PCR Primer, Index 36 (100% over 8bp)
ATAGGAGG83864812.037566761682792No Hit
AGCCATTG78979081.9188638032601057No Hit
CGAACGAG75385701.8315595853158249No Hit
ACTCGGAA69567651.6902051209366824No Hit
TTCACTTG57541561.3980210540198692No Hit
TGTACGCC55937751.3590551284063195No Hit
CTGATCCT54876801.3332784473906785No Hit
AGTTCGTC54286841.3189448683040224No Hit
GAGTAATT53728161.3053712633746493No Hit
CCTTACTG53236311.2934213500351488No Hit
TGCTGCCG52580401.277485459705756No Hit
TCCGATGC49625701.2056985146122876No Hit
CCAGATTC48222681.1716109525230611No Hit
TGGCCTAT45154171.097058896852855No Hit
GATTGGAT16588020.40302002942747245No Hit
ACTTCTAT10227950.24849672896359642No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)