FastQCFastQC Report
Tue 19 Sep 2023
EGAF00001841346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412826297
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGGAT113743962.7552498672341117No Hit
AACGAGAT112575602.726948375577925No Hit
TGTACCTA109590052.6546286124791125No Hit
TTGGAACG104819912.539080256314195No Hit
CACGGCAA103579422.5090315406917982No Hit
CTCTTAAT103166032.4990178859657286No Hit
TAATTGAT102754362.489045895252162No Hit
CTCAGAAC102109672.4734293997748886No Hit
CATAATAA101623072.461642359958479No Hit
GACGCTCA100783862.441313955346212No Hit
GAGCCGTC100426962.4326686727517264No Hit
GGTTGCAG98170732.37801541988494No Hit
GGATACTA97923682.3720310627401724No Hit
CACTCTCG96932962.3480325915381304No Hit
CGCGTGAA95677512.317621495900006No Hit
AGGCGTGC94871282.298091974504231No Hit
AACTACCT93678472.2691982240656534No Hit
AACTCTAC90956652.203266862139841No Hit
TGTAGACG90782602.199050803200165No Hit
GGTGCGGA90234812.185781541915679No Hit
GCCACGAC90082362.182088705458606No Hit
GAGTGGCA89798922.175222863770231No Hit
ACGCGACC88851732.152278831210212No Hit
ATTGAGTA87597682.1219016481404043No Hit
TATAATCC87427612.1177819977878007No Hit
TTGAGAGT86875022.1043964648405136No Hit
ACCAGCGG86801662.1026194462607113No Hit
CGCGCGCT86380102.09240788747525No Hit
ACCACCAA85542882.0721276871565184No Hit
GGAGAACC85210892.064085806045442No Hit
TTGTCACA83895652.032226401507557No Hit
ATTATCGT83550092.0238558107164377No Hit
CTATGGAG83195392.015263819300736No Hit
AAGACACT80703121.9548929074157309No Hit
CCGACCGC79870131.934715171499843No Hit
CCAATCTA79503211.925827171809261No Hit
GGCACGTT78993421.9134783945219458No Hit
TTCGTTCG78812371.9090927727406861No Hit
AGCTGTGG78709091.9065909941294268No Hit
CGACAGTT77278041.8719262934938468No Hit
CAATTAAG76740031.8588939357223166No Hit
CGCTGAAG58029801.4056711120803431No Hit
ATCTGTCT37716170.913608708410356No Hit
AGAGTATT34316420.831255669742376No Hit
TCGCGGAT25100500.6080160150262909No Hit
CTGCTCAG22037410.5338179801079872No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)