FastQCFastQC Report
Wed 20 Sep 2023
EGAF00001841348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405535067
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCAGGT155516523.8348476532610185No Hit
TCTCTATA137429873.388852929948966No Hit
CTTAGATT136155813.3574361647004003No Hit
ATCCTAAC130074523.2074789724657773No Hit
CAACGTAA127540993.1450052135688673No Hit
AGGTGTAA127453373.1428446112651462No Hit
CTCTCTGC122718223.026081589141587No Hit
ACGTGCGC121206802.9888118158718937No Hit
GTGGCGAA110104202.7150352450285147No Hit
ACGAGTTC108547402.676646456322358No Hit
TGCCTGAC108322892.6711103136242467No Hit
AGGCCGAC106720852.631605961710828No Hit
GCCTCATT104931432.587481047600749No Hit
ATATTACA103238132.545726335424404No Hit
ACATTCCT102638532.530940930935573TruSeq Adapter, Index 27 (100% over 8bp)
CTTCCTGT102481452.5270675297729555No Hit
GGATCTCT101448842.501604626955725No Hit
CGAAGCAA96311312.3749194049352087No Hit
CGCATATA95021832.3431224012990226No Hit
GTCCTCAA93349322.301880345158906No Hit
TAACATTA92614332.283756388445688No Hit
TGCATCAA90817072.2394381495003985No Hit
TAGCTGGT90073732.2211082919741685No Hit
CGCTACAA88669892.186491310257024No Hit
AACCTTCT86920662.143357432515201No Hit
CCTTAGCC85464692.107454988596584No Hit
TAATGATT84882662.093102838872378No Hit
AGTTCCGG81708562.0148334052699814No Hit
GCGCTGGC80700511.9899761220895849No Hit
AACGCTGG79059381.9495078584659116No Hit
TGATAAGG78922311.9461278794911219No Hit
ACATCCAG77138721.9021467260684513No Hit
CGTTCGCG73054501.8014348436111938No Hit
GGTCAACT71532171.763896043051685No Hit
TATTAGAG50973361.2569408701714075No Hit
TCGGTAAT43515421.0730371684478768No Hit
TATATAGC39310890.9693585881686527No Hit
AGGAATAC35978810.8871935605016371No Hit
TCAGGTAC34925800.8612276185723786No Hit
ACCTGAAC31125490.7675166103453145No Hit
GCCTGTGA29746670.7335165913038045No Hit
ATGCTAGG27383750.6752498668629315No Hit
AGCACAAC27288830.6729092554652001No Hit
CTCCTTCA25864940.6377978652090276No Hit
TATCTACT16192050.39927619872142894No Hit
TATGGTGG10945010.2698905937029608No Hit
NGCCAGGT4135310.10197169952752816No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)