FastQCFastQC Report
Tue 19 Sep 2023
EGAF00001841352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416520869
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAAC110657092.656699777508627No Hit
CAGAGGAT106451362.555726925653754No Hit
ATGCAACT105010572.52113586174238No Hit
GCCTCATT104564492.510426194275514No Hit
AACTACCT97949222.3516041401517387No Hit
CTCTATAG96658742.3206217789774177No Hit
AACTCTAC95279222.2875017097882795No Hit
CGACAGTT95039772.2817528981963204No Hit
TAGCTGGT95006082.2809440551704983No Hit
ATTGAGTA93034972.23362085610169No Hit
AAGACACT92656252.2245283945184506No Hit
TGTACCTA92410742.218634092017128No Hit
GGATCTCT91648392.2003312876023027No Hit
ACATTCCT90766372.1791553978535467TruSeq Adapter, Index 27 (100% over 8bp)
CGCGTGAA90254092.166856374248081No Hit
CCGTACAC88394592.122212752802069No Hit
ACTAAGTG87993882.112592346483364No Hit
TGCATCAA86055162.0660467795192274No Hit
TCAGGTAC85507202.0528911361701785No Hit
TTCGTTCG85457842.0517060815024855No Hit
CTTAGATT85440182.051282093142853No Hit
CGGCCAAG85318352.04835714966301No Hit
GTCTGGCG84907732.038498820091533No Hit
GATTGGAT83708792.0097141879342426No Hit
AGGCCGAC82696721.9854160056503676No Hit
TCGTCTGG81789681.963639425711464No Hit
ACGTGCGC81733281.9622853519015395No Hit
ATATTACA81255321.9508102966144536No Hit
ATATGGTT80533991.9334923168039393No Hit
CTATGGAG80373541.9296401688819103No Hit
ACATCCAG79882461.9178501233752108No Hit
GGTCAACT79521331.9091799695635416No Hit
TGATAAGG79010021.8969042341069349No Hit
GGAGAACC78269811.879132975687708No Hit
AGGATTGG77669251.864714490452098No Hit
GATGTCCA76506841.8368068851791435No Hit
GTTGGTCG75184161.8050514534963191No Hit
GAGACCAT72641601.7440086537416688No Hit
TATATAGC72322311.7363430114230365No Hit
CTGTTCGC72032281.7293798549143042No Hit
GAGTGGCA70675901.6968153401216495No Hit
TGTAGACG70240771.6863685646444766No Hit
GTTGTGTG66648221.6001171840443844No Hit
ACTTCTAT55741941.338274841638247No Hit
CTCCTTCA52183731.2528479095245526No Hit
TATTAGAG45754781.098499100653706No Hit
ACCTGAAC4880820.11718068320845647No Hit
ATAGGAGG4430590.10637138087791706No Hit
CCTTACTG4429990.10635697583738594No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)