FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001841358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407731600
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAAC115922702.843112969414193No Hit
AGCATAGT109571412.6873416237544503No Hit
ATGTTGTG106572322.6137861279331798No Hit
GAGAGCGG103547392.5395968818703283No Hit
GTCCAGAG98858962.424608737708826No Hit
CGAACGAG97205702.384060985216746No Hit
TGTAGACG96559582.3682142860646564No Hit
GAAGCGAT96420992.364815236297604No Hit
TCGAGCAA94764942.3241990564381076No Hit
ATGCAACT93621912.2961651733640465No Hit
GCCTGTGA92626032.27174028208753No Hit
CCAACAAT92459522.267656468127562RNA PCR Primer, Index 36 (100% over 8bp)
GAGTGGCA90245732.2133611915289375No Hit
CGACAGTT88528002.171232251804864No Hit
GAGTAATT87983502.15787787848673No Hit
AGCACAAC87323022.1416789868629262No Hit
CTGATCCT86952072.132581090109278No Hit
CAACCGGT85823362.104898418469405No Hit
GATATATG84492852.072266412512545No Hit
GGCGCCAT83292892.042836267780079No Hit
TATTAGAG82888322.0329138089861076No Hit
CTAACATA81815642.0066053256603116No Hit
CTCTTAAT80029471.9627978307298233No Hit
CCTTACTG79522641.9503673495014857No Hit
GTTGGTCG79408691.9475726188502438No Hit
TGGCCTAT79300001.9449068946336265No Hit
CAATATGG79185961.9421099566479516No Hit
TTGTACTC79008841.9377659224843011No Hit
CCGTGGCG78774111.932008949024309No Hit
ATGCTAGG78363571.921940070379632No Hit
CCAGCCAA78296571.9202968325241407No Hit
TATATAGC76445531.8748983399864028No Hit
TTGGAACG73957631.8138802584837674No Hit
TCGGCCAG73535301.8035222190284No Hit
TCGGTAAT66644331.6345147150723665No Hit
TGCTGCCG66268201.6252897739591436No Hit
TCTGCGCA59870931.4683907256636475No Hit
GATTATCT58713231.4399970470770478No Hit
CATTAAGT50976881.2502558055348174No Hit
CGTGCCAC49906271.223998090900975No Hit
CAAGCACT46764581.1469451963007038No Hit
TTCACTTG45316521.111430166315292No Hit
GTCATTAG40298970.9883700453926064No Hit
CTGGCCTC39076410.9583856144581386No Hit
GGCACGTT33390430.8189316207034235No Hit
ATTATCGT31212040.7655045623150132No Hit
CTACGGCC30799480.7553861412752899No Hit
CGTCCGAA30086010.7378876201893598No Hit
TTGAGAGT17486020.42886104486382703No Hit
GGTTGCAG14790390.3627481902310245No Hit
GGATACTA12584470.3086459327655742No Hit
CCGACCGC10523390.25809601218056194No Hit
TATGGTGG10403480.2551551069379955No Hit
TCCGATGC9891720.24260371283461962No Hit
CTATGGAG8059680.19767121312157312No Hit
AACTACCT7125300.17475466704076897No Hit
TCTACCGT7074660.17351267353327532No Hit
ATAACGCG6195920.15196075065067313No Hit
CAGCGTCT6181130.151598012025558No Hit
CGCTGAAG5872630.14403176010885593No Hit
GAGACCAT5713030.140117420381447No Hit
TATCTACT5629510.1380690140278556No Hit
TCAGGTAC4889430.11991785772797596No Hit
AGACAGAG4834830.11857874150544132No Hit
ATATGGTT4809550.11795872578922018No Hit
TGCCTAGA4800500.11773676604903816No Hit
CCAGATTC4717550.11570233948018746No Hit
TAGTCGTG4338280.10640038692120012No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)