FastQCFastQC Report
Thu 5 Jan 2023
EGAF00001841360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408701538
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAAGTG109129642.6701548649420546No Hit
TAGCTGGT97952252.396669473751772No Hit
ATCATGGA94464072.3113216177816294No Hit
CTCTCTGC93814632.295431293434477No Hit
ACAGCACG92532962.2640717344205346No Hit
GTCAACGT92246192.2570551227042364No Hit
ATTGAGTA91548492.2399839855753125No Hit
TTCGTTCG91205812.2315993829218232No Hit
TGGCTTCC89801612.197241792615911No Hit
CATCGAGC89457842.1888305201337412No Hit
TCCTGGAA88507942.165588620809154No Hit
GGATCTCT88137492.156524549217625No Hit
TCTCTATA87413402.1388077086218353No Hit
GTTCGGAC86915452.1266240011066464No Hit
CGAACTGC85874012.1011423255275345No Hit
ACGTGCGC84557612.06893300215572No Hit
GCTCCAGA84168642.059415788153946No Hit
CGGCCAAG84142152.058767637913807No Hit
TCGCGGAT83827202.0510615254890476No Hit
TAAGCAAC83669682.047207368228695No Hit
CGCTGAAG82921022.028889355439617No Hit
AGGATTGG81832892.0022652814191266No Hit
TCGTCTGG81535791.99499591802368No Hit
ACTCGGAA81137531.9852513987848022No Hit
GAGACCAT81117571.9847630228394197No Hit
AACTCTAC80395181.967087777389279No Hit
CTCTATAG79934621.9558189184010362No Hit
AGCCATTG77191551.8887022147687635No Hit
AGTTCCGG76567081.8734228497079939No Hit
AGGCGTGC76307941.8670822814471522No Hit
GCTCACGT76168121.8636612030562019No Hit
ACATCCAG75997561.8594879865609901No Hit
GGAAGGCG75683051.851792639938634No Hit
GATGTCCA75384361.8444843728481393No Hit
AACCTTCT74698801.8277102739946136No Hit
AGAGTATT72850311.782481914712051No Hit
AAGACACT72658841.777797077925359No Hit
CAGATTCA72602451.776417342476455No Hit
TGTACGCC70449711.7237446755093935No Hit
CCAGATTC70429201.7232428423110069No Hit
ATATGGTT67633851.6548469656113698No Hit
GTTGTGTG66661751.6310618826200747No Hit
ATCTGTCT66167901.6189784928090973No Hit
CTGTTCGC64456391.5771017235565188No Hit
TCCGATGC61495691.5046601072492172No Hit
CTGCTCAG55811671.3655850250311512No Hit
GGCGCCAT7255490.17752539017849256No Hit
AGCTGTGG6502020.1590896875949608No Hit
CGTTCGCG5492580.134390979463356No Hit
GCCACGAC4755420.11635434560072538No Hit
GGTCAACT4678420.11447033018016195No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)