FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001841362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415075561
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTACCT101046262.434406394743149No Hit
AACGAGAT100072732.410952110957937No Hit
ACCAGCGG97104922.3394516354095827No Hit
GGTGAGAT94379462.273789855818565No Hit
GCAACAGC92362292.225192198198342No Hit
CGTTCGCG88479922.13165814404573No Hit
CTCACGCC86022512.0724542247863154No Hit
AGGTGTAA85569432.061538621879981No Hit
TTGTCACA85497382.059802793352124No Hit
GCCACGAC84994072.0476770493360847No Hit
CACTCTCG84942202.0464273973480216No Hit
ACGAGTTC84507862.035963278502923No Hit
GCCGTCCT84290042.0307155592810244No Hit
CTGTAGCA83940452.0222932373510663No Hit
CATAATAA81666141.9675005631083158No Hit
TAATTGAT81215301.9566389262797381No Hit
TGAGGCGG80690491.9439952042852267No Hit
CTCACCTT80003521.9274447237330843No Hit
CACGGCAA79756201.9214862905407242No Hit
CAGAGGAT79485571.9149662728517036No Hit
GACGCTCA79070081.9049562881877307No Hit
ATAGGAGG78611781.893914925046623No Hit
CTTAGATT78401361.888845486617315No Hit
ATATTACA77975501.8785856679237254No Hit
AGCTGTGG77728101.872625307371445No Hit
GTCTGGCG75979171.8304900875626355No Hit
TGCATCAA75668811.8230128947533966No Hit
GAGCCGTC75352451.8153911499501654No Hit
TCAGGTAC74804811.802197407618513No Hit
ACCGTTCA74521631.7953750353420592No Hit
AACGCTGG74471161.7941591121525942No Hit
CGCGTGAA74302261.7900899735217124No Hit
GAGGTGCG73892301.7802132176122025No Hit
GGTCAACT72893211.7561431423325835No Hit
CCGTACAC72833831.7547125594320403No Hit
AAGCGCAC72381741.7438208076047148No Hit
ACCACCAA71595031.7248673910724415No Hit
ACATTCCT71317271.7181755974305606TruSeq Adapter, Index 27 (100% over 8bp)
GGTGCGGA71156211.7142953400718284No Hit
CGCGCGCT70582231.7004670144865504No Hit
AGGCCGAC70471221.6977925616777036No Hit
CCTTAGCC67525941.6268348788667903No Hit
TAACATTA65549451.5792172837658345No Hit
GGATCAGA64507251.5541086024093815No Hit
CTATGGAG60378221.4546320157837478No Hit
CTCCTTCA47907811.1541949105502745No Hit
TAATGATT10537920.2538795580884609No Hit
CCGGTCTT9264490.22320008380353668No Hit
TAGCTGGT4271690.10291355120278932No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)