FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001841366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397372958
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACAT118681792.986659952839569No Hit
GGAATATC104588362.6319949028841565RNA PCR Primer, Index 37 (100% over 8bp)
ACAGCGGA98322102.47430274306688No Hit
CGAGGAGT95393932.4006145380431247No Hit
TTAGAGAT92880302.337358346362361No Hit
TAGGATCA91334082.2984472939399163No Hit
GCGGAAGT89051082.240994969768426No Hit
CCATCTCC81801272.058551503144811No Hit
TTACAGCG78488421.9751827199071759No Hit
CGAGCCTT73771681.8564846579217906No Hit
CTGAACTA73553961.8510056741203813No Hit
GCACCACT73083151.8391576107199523No Hit
TGTCCTGC71304571.794399154861464No Hit
CAGGCTAG71160271.7907678055938574No Hit
TTAATACT70917871.7846677427908921No Hit
CGGCACTC70171031.7658733083694134No Hit
GGCACTCC69566031.7506483166375906No Hit
GACGCCTC69010941.736679323810454No Hit
GAGTATAA67847061.7073899628570095No Hit
CTAATGAT67816411.7066186471601825No Hit
CTTATGTA67736431.704605928418511No Hit
AGGTTATC67601771.70121717240759No Hit
GTTGCCTT67557821.7001111585454187No Hit
ACAGGCTG66708101.6787277205712623No Hit
GTTGCAAC66505841.6736377919304717No Hit
AATCTGTA65340921.6443222590904134No Hit
TGACGGAA65303941.643391647199103No Hit
GTAGCTTC63391391.5952617993698504No Hit
TCGCACGC63247571.5916425294345269No Hit
TACTGCAC62264111.5668934875029923No Hit
ATGTGCAT61604041.5502826440444395No Hit
AGGCCAGA61426571.5458165625855196No Hit
ATCATCTC61198091.5400668004187643Illumina PCR Primer Index 7 (100% over 8bp)
GGCTCTTA60881051.5320884014457774No Hit
TGGCTGTG59424051.4954225949114535No Hit
ATAACTGA58561551.4737175447152595No Hit
GCGTACGA57269371.441199478903645No Hit
CACTGCTT56139901.412776055083245Clontech Universal Primer Mix Long (100% over 8bp)
TTAACCAG54833331.3798958609558933No Hit
AGATGGAC54814151.379413190970081RNA PCR Primer, Index 16 (100% over 8bp)
AGTGGATG50493321.2706783132434494No Hit
GCCATGCG49966501.2574207427572361No Hit
TCTGACCG49774031.2525771821644693No Hit
GACTCGGT49430081.243921585625361No Hit
TACTCCGG47654861.1992476850928544No Hit
GGAAGAAT47120851.1858091762751506No Hit
ATGGTCAA44285651.1144605869229782No Hit
GGATTGTG43882081.1043046366532068No Hit
CTGGATGC43815071.102618311535935No Hit
GAGTGAAC43082181.0841749327089338No Hit
CTATCCTG40481281.0187225674274494No Hit
CGTCGTGG26621110.6699275696561113No Hit
GCGGAAGG9556800.24049950575650397No Hit
CGAGGAGG5490470.13816919066747366No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)