FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001841368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397855534
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCCGA152932983.8439324561462556No Hit
CGCAAGTG132378103.327290654200125No Hit
GTTACCAA126087213.1691706970198887No Hit
GCTGGAAT103979222.6134918610934794No Hit
GCGAGATA98863942.484920569183285No Hit
AATTAACA85612942.1518599763903246No Hit
TGGAGTTA82544402.074732985868182No Hit
TGTGAGTC82196812.065996397576815No Hit
ATTGACCT79670472.002497469345242No Hit
GGCGGAAC78611391.9758777566733559No Hit
AGAGCCGC78474561.972438568618729No Hit
CTTGCGAT76144511.9138733407689636No Hit
TACTGTTG73816211.8553520987344112No Hit
CGTAATGT72413461.820094325997235No Hit
TCTATGAA69799071.7543822828916589No Hit
CCGAAGGT66711831.676785272515526No Hit
GTGCGGTG66187451.6636051114975818No Hit
GTACTATA66064401.6605122803193182No Hit
ACTCTTGT65386841.6434819780589003No Hit
AAGCAGGA65256861.640214963052393No Hit
CCTACTCG63742811.6021596924676684No Hit
ATTAATCA63269461.590262157821336No Hit
GACCACTT62622781.5740080166887913ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TCTAGTGA62537871.5718738249346558No Hit
CTGACTGG61872451.5551486585580583No Hit
TCGTGAAG60491861.5204478719152366No Hit
ATATACAA60370671.5174017913748563No Hit
TAACCTCT59174691.487341131215734No Hit
TTCCGGTT58637131.4738296941723577No Hit
AACGGATA58180191.4623446207989657No Hit
TCATTCAA56882801.429735045485128No Hit
GTGGTACC54095151.3596681553259482No Hit
TCGATTGC50883581.2789461412895669No Hit
AACCAACG50794081.2766965810258153No Hit
GGCTGAGT50160341.2607676835783312No Hit
TCCGACTT48305311.2141419654099872No Hit
CGGAGAGA44640791.1220351656588996No Hit
AGCTTCAC44462811.1175616825779782No Hit
CCGACACA44161601.1099908440635138No Hit
CTTAACAC42172481.0599948070597907No Hit
ATTCGATC41846181.0517933376289292No Hit
GTTATCCG37116910.9329243111646651No Hit
GCGCGAGG37095580.9323881869140973No Hit
AATCGCGG33027840.8301465526428998No Hit
TCTCCACG32939360.8279226298257296No Hit
TGATCCAT26534710.6669433433091319No Hit
TCGTGGAG8735840.21957316798313026No Hit
GCTGGGAT6019400.15129612348184657ABI Dynabead EcoP Oligo (100% over 8bp)
GCGAGGTA5871520.14757919642258893No Hit
GCGCGGGG5334410.1340790700174099No Hit
CTTGCCGG4826980.12132494303824364No Hit
TGGAGGTA4733610.11897811128599256No Hit
CGCAAGGG4567650.1148067479187056No Hit
GTTGCCGA4548640.11432893629173449No Hit
CCGAAGGG4457580.11204016581556461No Hit
CTTGCGGA4093030.10287729213790452No Hit
GTGCGGGG4042280.10160170349672704No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)