FastQCFastQC Report
Tue 8 Aug 2023
EGAF00001841370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403307453
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGAC166489594.128105958904756No Hit
TGTGAACA142093913.523215575190474No Hit
AGCGAAGA129050013.1997923430390958No Hit
ATTCTGGC101012512.50460310734699No Hit
TGTGGAGC97616622.420402084659715No Hit
CGCACCGA97542732.4185699836298338No Hit
TCGGATTG97244832.41118355925845No Hit
CCAGCGTG96905252.4027636801445373No Hit
ACACTAGC92292942.2884015485823417No Hit
AGCAAGCT87324292.1652039740510323No Hit
TCCTTACA87282602.1641702713587097No Hit
GCAGCTGG84793732.102458790911558No Hit
ACGTGGTA83362892.0669811425478417No Hit
TTCGCATA82766002.0521812672774984No Hit
GGAGTGCA80235261.9894316210417267No Hit
CGTGTGGT79370631.9679931379795255No Hit
AAGTGAGA79144861.9623951754742308No Hit
TCCAACCA78652861.9501960455960132No Hit
TCGGCAGC78346281.9425944008032006No Hit
GCATCCGT77539261.9225843565058045No Hit
GGCCGCTC75357191.8684799757469395No Hit
CAAGACGC74289201.8419991856684086No Hit
GGTAATTA67583311.6757267810768675No Hit
CGCCTCAT67332101.6694980342949428No Hit
GTCTTGTA67091391.6635296347970043No Hit
GCCTTAGG67017551.6616987735160946No Hit
CATCTCCG66319411.64438840657874No Hit
TGCAGTGT64265231.5934550557388287No Hit
CGTGATCC64252001.5931270181610058No Hit
GATGGCGT63952571.585702657471098No Hit
TAATTAGA63327251.5701978609356373No Hit
ACCTCGCA62019991.5377843761295429No Hit
TTCTTGCC60272761.4944618442248325No Hit
TCACGCCG58229381.443796279162736No Hit
CCGGTAGG57608551.4284028120849035No Hit
TTACAATT57171841.41757459661922No Hit
CAGGCGCA56174991.392857721377145No Hit
CGATGCTC49607551.2300181816873095No Hit
TAATACCA46690401.1576875074510462No Hit
ATAATATG42576261.0556774907901343No Hit
GGTCCGTG42323601.04941279128804No Hit
GTTCAGGA39744430.9854623241986059No Hit
TCACCGAG39325030.9750633098268084No Hit
GTGTCGGC37030600.9181729651794954No Hit
CGCTTACC34920240.8658466323953602No Hit
GACTTATC32320110.8013764625371305No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)