FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001841374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409573278
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTAT78947111.9275454293675869No Hit
CCGCTC73462931.7936455805595795No Hit
AACATA67752621.6542246196051884No Hit
TGTGAT65408841.5969996948873213No Hit
AGAGGT63331271.5462744617826363No Hit
CCTGAC62613291.528744509547813No Hit
CATGAG59915631.46287937271142No Hit
GACTAA59678381.4570867584774414No Hit
CTGAGG59407881.4504823237027686No Hit
TTCTAC59277081.4472887559817806No Hit
TGCGGA58730591.4339458444845126No Hit
ACCAGG57482741.4034787689444916No Hit
AGTTCC55353301.351487095796323No Hit
TACAGT55004021.3429591956924494No Hit
TCATGT54956321.341794568931814No Hit
CTGTAT53929451.3167228649130767No Hit
TGCGAC50724481.2384714219563906No Hit
GACACG50620651.235936344460441No Hit
CGCTTG50388271.230262634468062No Hit
ATGTGA49951591.2196008060857915No Hit
TTCCAT49867371.2175445195914367No Hit
AGTTAG49683791.2130622935805886No Hit
AAGCGC49086101.1984693005289275No Hit
CGTACT48785591.191132151936924No Hit
GTTGGC47728261.1653167470559445No Hit
AGGTGT47676521.1640534810476575No Hit
GTCGAG47372301.1566257503742712No Hit
GCGGCT46928591.1457922799348252No Hit
AACTCT46265881.129611780971707No Hit
CGATGT46264281.1295727159231321No Hit
ACTAAT45732951.11659994576111No Hit
CCGGAA44949641.0974749187616677No Hit
CTAGTT44551221.0877472333534417No Hit
CAAGGA44457531.0854597306028348No Hit
ACAAGT44453961.0853725667132026No Hit
GGCAGG44080141.0762455064268133No Hit
ATACCT43944861.072942556569816No Hit
TCGGAC43422251.0601826909225265No Hit
GATTCA43384131.0592519661402324No Hit
ATTCAT43041941.0508971730328558No Hit
CCAGCG42916851.0478430187039693No Hit
CGACAA42784131.0446025729246915No Hit
CTCCGC42779651.0444931907886823No Hit
TCTTCA42643841.0411773006343445No Hit
ACTTGA42423961.0358087863339562No Hit
GCTCAA41862241.0220940244055667No Hit
TGATAA41851181.0218239872572936No Hit
GTCTGC41469621.0125079497984242No Hit
CAGATC41145841.0046026489062112No Hit
CAGTCC40722440.9942650604271112No Hit
ATTGAA40671670.9930254776045228No Hit
GCCATA40310240.9842009272880348No Hit
CCGTCG39560720.9659009052831812No Hit
AATTGC38965430.9513665098043822No Hit
TGACAC38311110.935390858189728No Hit
GGTAAT37910140.9256009128603356No Hit
CTACCG37793390.9227503850971449No Hit
ATTCGA37538600.9165295202681655No Hit
ATTGCT36776040.8979111181174275No Hit
TACGAA36593220.8934474480046523No Hit
GCCTGG36570160.8928844229920683No Hit
CGTCTC36094430.8812691632680195No Hit
ATCAGC35600770.8692161308433798No Hit
TTCACA35316000.8622632846667305No Hit
GACCAT35163600.8585423387899832No Hit
TAGCTT35070990.8562812049471645No Hit
AGAACG34804970.849786152308501No Hit
AGGTCA34738860.8481720333327019No Hit
TTCTGT33185550.8102469517066492No Hit
GACTTG33026940.806374384610121No Hit
GAACCG32402640.7911316909693508No Hit
GCGCAG32370370.7903437977709084No Hit
TTAGGC32155980.785109325418442No Hit
TTAGCG31658740.7729688849476162No Hit
AGTGCA31196020.7616712728997911No Hit
GCCAAT31039900.7578595007851073No Hit
TACTCG31019970.7573728967737978No Hit
TATAAG30785580.7516501113141468No Hit
GATCAG28403760.6934964150664146No Hit
TCTCGC28386750.693081104768754No Hit
ACAGTG27408600.6691989314791186No Hit
CTTAGC27362240.6680670216966644No Hit
TGACCA26709560.6521314117567992No Hit
GGCTAC25923120.6329299637560827No Hit
TAGAGC25236280.616160315029146No Hit
CCACTT24939050.6089032497867207No Hit
GTACTT24842680.6065503130797513No Hit
CGAAGC24753270.604367309334082No Hit
CTTGTA23635060.5770654793548323No Hit
GTGGTG21862730.533792880892No Hit
CTTCAC20611900.5032530466990085No Hit
GGCCGT17515750.42765851535851424No Hit
TCCAGA11232920.274259103397854No Hit
GTCTCA7092100.173158269373228No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)