FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001841376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409583227
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTAT79551071.942244329258141No Hit
CCGCTC74094681.8090262275315294No Hit
AACATA68349851.6687658452380911No Hit
TGTGAT65849961.6077308751708232No Hit
AGAGGT63766511.5568633136434564No Hit
CCTGAC63035881.5390249366827708No Hit
CATGAG60531441.477878878082085No Hit
GACTAA60182721.469364857560439No Hit
CTGAGG59977741.464360258092307No Hit
TTCTAC59630431.4558806628084895No Hit
TGCGGA59277211.4472567745065401No Hit
ACCAGG57975451.415474223020368No Hit
AGTTCC55806331.362515023106647No Hit
TACAGT55307051.3503250708066714No Hit
TCATGT55244671.3488020592210432No Hit
CTGTAT54247861.3244648809800994No Hit
GACACG51142651.2486509854076617No Hit
TGCGAC51138161.2485413617779813No Hit
CGCTTG50770891.2395744418508623No Hit
ATGTGA50425501.2311417234866406No Hit
TTCCAT50097301.2231287000431783No Hit
AGTTAG50044791.221846665122349No Hit
AAGCGC49443101.2071563662932907No Hit
CGTACT49072721.198113515522451No Hit
GTTGGC48164781.175946103867188No Hit
AGGTGT48062231.1734423392293847No Hit
GTCGAG47878201.1689492353162205No Hit
GCGGCT47172371.1517163519003184No Hit
AACTCT46566531.1369247305627581No Hit
CGATGT46555931.1366659309024878No Hit
ACTAAT46035261.123953740420137No Hit
CCGGAA45310911.1062686900506304No Hit
CAAGGA44862221.0953138957518882No Hit
CTAGTT44705281.091482195876151No Hit
ACAAGT44688651.091076173390274No Hit
GGCAGG44470241.085743679635592No Hit
ATACCT44126851.0773597913959498No Hit
GATTCA43745491.0680488632411698No Hit
TCGGAC43691521.0667311823293975No Hit
CCAGCG43265421.0563279242877786No Hit
CTCCGC43151701.0535514434041997No Hit
ATTCAT43146441.0534230201765562No Hit
CGACAA42991161.0496318493042196No Hit
TCTTCA42990491.0496154912124855No Hit
ACTTGA42830991.0457212887772869No Hit
GCTCAA42300531.03277007483512No Hit
TGATAA42140781.0288697686343489No Hit
GTCTGC41780211.0200664296245705No Hit
CAGATC41399631.0107745452183763No Hit
CAGTCC40990451.0007843900306983No Hit
ATTGAA40972231.0003395475957808No Hit
GCCATA40509720.9890473371361957No Hit
CCGTCG39905440.9742938032958073No Hit
AATTGC39246320.9582013474394546No Hit
TGACAC38528350.9406720651673561No Hit
GGTAAT38264420.9342281977772492No Hit
CTACCG38014460.928125408807329No Hit
ATTCGA37766790.9220785303300518No Hit
TACGAA36880190.90043213610405No Hit
ATTGCT36875390.9003149438050596No Hit
GCCTGG36856500.8998537432784082No Hit
CGTCTC36363760.8878234654858072No Hit
ATCAGC35815970.874449138514161No Hit
TTCACA35592220.8689862683268521No Hit
GACCAT35403980.8643903770014487No Hit
TAGCTT35189370.8591506604834676No Hit
AGAACG35097320.8569032539997055No Hit
AGGTCA34926800.8527399975780746No Hit
GACTTG33321510.8135467422351257No Hit
TTCTGT33271000.8123135374388756No Hit
GCGCAG32584210.7955455168089683No Hit
GAACCG32561090.7949810405688316No Hit
TTAGGC32339740.7895767665310182No Hit
TTAGCG31941440.7798522472210513No Hit
AGTGCA31359370.7656409719141161No Hit
TACTCG31301020.7642163530295151No Hit
GCCAAT31228860.7624545621346941No Hit
TATAAG31023160.7574323838217134No Hit
TCTCGC28668610.6999458989076229No Hit
GATCAG28491430.6956200381711432No Hit
CTTAGC27606850.6740229623709664No Hit
ACAGTG27525860.6720455864761279No Hit
TGACCA26849650.6555358772052451No Hit
GGCTAC26068620.6364669811051613No Hit
TAGAGC25454340.6214692966418764No Hit
CCACTT25088880.6125465679775016No Hit
GTACTT25073720.6121764356331906No Hit
CGAAGC24917900.6083720806272177No Hit
CTTGTA23761860.5801472920178931No Hit
GTGGTG22083090.5391600178979008No Hit
CTTCAC20703710.5054823692768063No Hit
GGCCGT17669350.4313982808675903No Hit
TCCAGA11389050.2780643651699145No Hit
GTCTCA7157270.1747451928738283No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)