FastQCFastQC Report
Tue 8 Aug 2023
EGAF00001841378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414283535
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAG84743532.045544243026699No Hit
GAAGAT74567961.799925744092147No Hit
GGTACC61594101.4867619588116143No Hit
CGAGCC60647131.4639039420188398No Hit
GAATAC59517271.436631315796801No Hit
GGTGGT59374341.4331812631655756No Hit
ATGCCG58334991.4080933725739306No Hit
GGACTA58248461.4060047064144126No Hit
TTACGT56021691.352254802981731No Hit
AACCTT55972761.3510737278033509No Hit
CAGCCT50857391.2275986300058968No Hit
GAGAAG49225161.188199767581881No Hit
GTACGC48409811.1685188019842498No Hit
GGAGGC47574641.1483594200768805No Hit
GCACAT47131861.1376715707516594No Hit
ACACGA44363921.0708588744662517No Hit
AACAAT43812141.0575399768180505No Hit
TTGAAT43404771.0477068561269276No Hit
AGACGG43365971.0467702994761787No Hit
TTCGTT42849471.0343029925145348No Hit
CAACGG42736021.0315645298334148No Hit
CACATT40292300.9725778747156824No Hit
CCTTAT40010560.9657772182522291No Hit
TATTCT39756960.9596558067411489No Hit
ACGCTA39023170.9419435411547312No Hit
AGCCTA38074420.9190425586187005No Hit
CGTCAG38039250.9181936231185245No Hit
TATGGT37746800.9111344480537948No Hit
GTTACA37694010.9098601999715001No Hit
TTAGTA37661770.9090819889812904No Hit
CGAATT37435090.9036103739918122No Hit
AGGCTT36320580.8767082669602112No Hit
TGTGTG36294740.8760845395412588No Hit
TCTCTG36091170.8711707550723684No Hit
CCGACT35641960.8603276980341495No Hit
CTCGTC35313390.8523966563141352No Hit
TAATGA34827530.8406689394498866No Hit
CGTGAA34237690.8264313473138631No Hit
CAGGAC33622030.8115705105200476No Hit
GAGTCG33461010.8076838004194398No Hit
CACCTA33328500.8044852663526683No Hit
CGAGAG33024060.797136675972411No Hit
TATTGG32583630.786505551083511No Hit
AGTAGG32284550.7792863406941819No Hit
GGCCAG32228330.7779292990729163No Hit
ACGAAC31522020.7608803473205856No Hit
GCATAA31475000.7597453758330028No Hit
GATCCT31295940.7554232151659129No Hit
CATACG31210300.7533560318780229No Hit
AGGACT30737520.741944040812532No Hit
GCTTCT30455400.7351342118870353No Hit
GGATTC30200860.7289901106014266No Hit
GAGATA29047380.7011473434492153No Hit
TAAGTC28703290.6928416790689015No Hit
GACCGA28300960.683130214190144No Hit
AAGAGG28297680.6830510413598745No Hit
TCCACG27549560.6649928774021878No Hit
TCGTGG27478220.6632708683438264No Hit
GAGCAC27359950.6604160602231031No Hit
GTAAGA26895420.6492032081361863No Hit
CCTCGG26620360.6425637939002331No Hit
GGATCG26586860.6417551689569319No Hit
TGCATA26564040.6412043384731667No Hit
CTATGC26519530.6401299535111865No Hit
GAGCCA26397060.6371737655468254No Hit
CTGGAG26264880.6339831970391969No Hit
ATCCAC26092110.6298128647569834No Hit
GAGTGA25930380.6259090166351893No Hit
CATTAA25803000.6228343108060039No Hit
CTGTTC25714850.6207065409925113No Hit
CAAGCT25143680.6069196063995157No Hit
AAGCAT24906210.6011875417641205No Hit
ACCGCT24814810.5989813232620987No Hit
CTATTA24544980.5924681510695325No Hit
TCAATG24234430.5849720771548403No Hit
ACGTAA24185440.5837895536929798No Hit
ACCTGC24156900.5831006535174033No Hit
AGTCAA24114570.5820788895218827No Hit
AAGGCG23698540.572036733248402No Hit
ATCCGG23403810.5649225234114119No Hit
AAGTTG23327210.5630735481679232No Hit
CCATGA23221900.5605315692789963No Hit
AACTAG23070160.5568688603567119No Hit
CATAAC22054060.532342179613776No Hit
ACAGCC22032010.5318099354346776No Hit
TCAAGC21698600.5237620655138998No Hit
TGGCAT21500550.5189815231252191No Hit
AATCGG21214840.5120850385714701No Hit
CCTGCT20057860.48415778821622735No Hit
CGTTGC18955470.4575482344476954No Hit
TACGGC17344600.4186649609427514No Hit
AATGTA10880180.2626264159882675No Hit
ACTAGC8887910.21453688715869437No Hit
GGGGGG6198070.14960937320378903No Hit
GAGTGG5863100.1415238479125172No Hit
GAGTAG5565460.134339396326721No Hit
GGAGGG4958010.11967673298915922No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)