FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001841380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001841380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414904809
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAG85226682.0541261067909193No Hit
GAAGAT75665961.8236944561421073No Hit
GGTACC60783011.464986876061974No Hit
CGAGCC60539301.459112998615545No Hit
GAATAC60200991.450959080110349No Hit
GGTGGT58991671.4218121535438748No Hit
GGACTA58469671.409230954466956No Hit
ATGCCG57869621.3947686010069842No Hit
AACCTT57066291.3754068104811965No Hit
TTACGT56450061.3605544880536682No Hit
CAGCCT51129601.2323212190100212No Hit
GAGAAG49696671.1977848634673212No Hit
GTACGC48145871.1604076153284597No Hit
GCACAT47472161.1441699148876339No Hit
GGAGGC46907571.130562215295991No Hit
ACACGA45025981.0852122950447654No Hit
AACAAT45016511.0849840499197492No Hit
TTGAAT43816701.0560663325548487No Hit
AGACGG43557631.0498222497102945No Hit
CAACGG42927681.034639249023503No Hit
TTCGTT42774121.0309381591188065No Hit
CACATT40941140.9867598329042264No Hit
TATTCT40589360.9782812616182524No Hit
CCTTAT40395370.9736057313329429No Hit
ACGCTA39460690.9510781544110759No Hit
AGCCTA38533770.9287376083413871No Hit
CGAATT38146730.9194092035698722No Hit
TTAGTA38121460.918800148204597No Hit
GTTACA37986950.9155581997604659No Hit
CGTCAG37940310.914434086494283No Hit
TATGGT37881060.913006048093311No Hit
AGGCTT36437700.8782183095882121No Hit
TGTGTG36068650.8693234982484862No Hit
CCGACT35554850.8569399348658792No Hit
TCTCTG35424630.8538013836325526No Hit
TAATGA35147560.8471234663370701No Hit
CTCGTC34918180.841594969317408No Hit
CGTGAA34362640.8282053920469261No Hit
CACCTA33766770.8138437845872256No Hit
CAGGAC33761230.8137102599839956No Hit
CGAGAG33391290.8047939979408626No Hit
GAGTCG33373750.804371250370347No Hit
AGTAGG32532900.7841051560335133No Hit
TATTGG32175150.7754826963213145No Hit
ACGAAC31977240.7707126865333586No Hit
GGCCAG31976900.7707044918826188No Hit
GCATAA31857670.7678308206834981No Hit
CATACG31626010.7622473713000516No Hit
GATCCT31262070.7534757207405615No Hit
AGGACT31095960.7494721518159121No Hit
GCTTCT30447440.7338415785872465No Hit
GGATTC30240600.7288563387078023No Hit
GAGATA29450520.7098138985417255No Hit
TAAGTC28755270.6930570428746224No Hit
AAGAGG28621670.6898370271721772No Hit
GACCGA28410860.6847561026943894No Hit
TCCACG27449250.6615794612301059No Hit
GAGCAC27416520.6607906055868348No Hit
GTAAGA27149340.6543510562202233No Hit
TCGTGG26776360.6453615243587114No Hit
GAGCCA26457520.637676870117936No Hit
TGCATA26438050.6372076058535151No Hit
CATTAA26408740.6365011787559204No Hit
GGATCG26392820.6361174762859882No Hit
CTATGC26301580.6339184176580609No Hit
ATCCAC26241520.632470856706797No Hit
CTGGAG26165360.6306352549410918No Hit
CCTCGG26136440.6299382275899337No Hit
GAGTGA26077650.6285212760693742No Hit
CTGTTC25529220.6153030634070091No Hit
CAAGCT25363910.611318776013512No Hit
AAGCAT25347890.6109326633521859No Hit
CTATTA25194690.6072402501365078No Hit
ACCGCT24916820.6005430513098728No Hit
ACGTAA24560000.5919430063776387No Hit
AGTCAA24535650.5913561247731887No Hit
TCAATG24200870.5832872860242023No Hit
ACCTGC23919990.5765175404366065No Hit
AAGGCG23812250.5739208002286617No Hit
AACTAG23569300.5680652402368275No Hit
AAGTTG23514980.5667560242715818No Hit
ATCCGG23122400.5572940948968369No Hit
CCATGA23060970.5558135143234746No Hit
CATAAC22491970.5420995252913542No Hit
ACAGCC22228370.5357462607766497No Hit
TCAAGC21301230.5134004122858937No Hit
AATCGG21271320.5126795240399347No Hit
TGGCAT21189380.5107046132116536No Hit
CCTGCT19887690.4793313928544993No Hit
CGTTGC18405010.44359596709326166No Hit
TACGGC17255580.4158925041526814No Hit
AATGTA11135530.2683875857413839No Hit
ACTAGC8984630.2165467790468536No Hit
GGGGGG5686960.13706662050282478No Hit
GAGTGG5268340.12697707728907043No Hit
GAGTAG5093200.12275586808153868No Hit
GGAGGG4687940.11298832643802882No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)