FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001848626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001848626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126354
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA469781.502644934002995Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT404811.294830975634877Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG200650.6418019200640747Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT197130.6305427984163021Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG154460.494057934578106Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC137130.43862595214745354Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC128790.4119495105160836Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG128150.40990239748921586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA120130.3842495123712798Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC99250.31746244986972044Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT94290.30159732391149563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG89530.28637192077416695Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC83230.26622065191593786Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC69840.22339120905693982Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA64960.20778197222707345Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG54020.1727891339240534Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT53690.17173359126957471Clontech Universal Primer Mix Long (100% over 23bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA51460.1646006818165825Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50740.16229767966135633Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC50380.16114617858374322Clontech Universal Primer Mix Long (96% over 26bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA48750.1559324375934395No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC48190.15414121369493025Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG47070.15055876589791176No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC46620.1491193895508954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC44640.1427861336240234Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG43190.13814814317252622Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT42320.1353653489016279Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG40600.1298637326419209Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG38950.12458601936952757No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA38920.12449006094639316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38630.12356246285609371Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC38000.1215473359702708Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT37640.1203958348926577Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37300.11930830609713423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT36950.11818879116056594No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG36730.11748509605758017Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC35770.1144144265172786Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA34720.11105588170757373Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG34500.11035218660458797Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT34120.10913671324488526No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG32900.10523440403741866Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31880.10197181765084826No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)