FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001849161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001849161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888055
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.22768860036822045No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17150.19311866945177944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.1832093732933208No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14340.16147648512761034No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT13860.1560714144957238No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG13790.15528317502857367No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC12960.14593690706093654No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11960.13467634324450625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC11710.1318612022903987No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11580.13039732899426273No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11280.12701915984933365No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11110.1251048640005405No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10990.12375359634256887No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG10800.12161408921744711No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT10530.11857373698701094No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA10290.11587120167106768No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG10110.11384430018411022No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT9890.11136697614449556No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC9750.10979049721019532No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9530.10731317317058066No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC9380.1056240885981161No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA9360.1053988773217875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9250.10416021530198016Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9230.10393500402565156No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC9120.10269634200584424No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG9120.10269634200584424No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA9110.10258373636767994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)