FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001849720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001849720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379925
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA34890.25283982825153545No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA29970.21718571661503341No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGA23440.16986430421943222No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC23240.1684149500878671No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA22650.16413935539975No Hit
GTTATAGCCTCTTGTGATGCAGCGAGCGCCGGGCGAGGTCCGCGCTTCGG22500.16305233980107614No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGC20570.14906607243147274No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTT20530.1487762016051597No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGAT20410.14790658912622062No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT19860.14392086526441655No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAA18840.13652915919343442No Hit
GGCTATAACACTCCCGAGGGAGCTACATTCCTCAAGTCTTATCCAGCGGC18750.13587694983423013No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGA18180.1317462905592695No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG18180.1317462905592695No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTA18090.13109408120006522No Hit
GTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG17820.1291374531224523No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAA17720.12841277605666973No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGA16930.12268782723698751No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT16850.12210808558436148No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACT16650.12065873145279635No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTC16510.11964418356070075No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAAT16500.1195717158541225No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATAAGCGTACATTCCGG15770.11428157327390981No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTC15610.11312208996865772No Hit
CATCAGGATCGGTCGATGATGCACCTTGCGGTTCTCACCTCCGTTCACTT15300.11087559106473178No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGG15150.10978857546605793No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT14760.10696233490950595No Hit
GTGCATCATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCA14580.10565791619109734No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCT14170.10268674022138884No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)