FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001849973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001849973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658175
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA154710.9330137048260889Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT149530.9017745412878617Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG68610.4137681487177167Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50340.30358677461667194Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49120.29622928822349875Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT47240.28489152230614984Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC45350.27349344912328316Clontech SMART CDS Primer II A (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTG43930.2649298174197537No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA43580.26281906312663017No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGC35420.21360833446409455No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34920.2105929711882039Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGG34120.20576838994677882No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG32290.194732160357019Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29560.178268276870656Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGG28900.17428799734648032No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG28490.17181539946024998No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT27430.16542282931536176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.15999517541875857No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGG25850.15589428136354727No Hit
CTCCACGGCAACGTAACTGAAAGCGAAGACGACGGCGGGGGCCCTGGGAA25640.1546278287876732No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC25390.15312014714972785No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC25370.15299953261869226Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT24970.1505872419979797No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA23410.14117930857720085Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAG23030.13888763248752395No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT22270.13430428030817013No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA21350.12875601188053132No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGG20930.12622310672878315No Hit
CCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAA20330.12260467079771434No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGAATTTTCAAGGGAC20290.1223634417356431No Hit
TCCCAGGGCCCCCGCCGTCGTCTTCGCTTTCAGTTACGTTGCCGTGGAGA20210.1218809836115006No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT19270.11621210065282615Clontech Universal Primer Mix Long (100% over 23bp)
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAG19240.1160311788562727No Hit
GCCGTGGAGAATCCACATCCAGGTGCCGGAATGTTAACCGGCTTCCCTTT18580.11205089933209703No Hit
TGCCAAGGGCGTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGC18170.10957830144586668No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA17900.10795000527688574No Hit
ACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTT17690.10668355270101165No Hit
ACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG17460.10529648559410194No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG16940.10216050778717567Clontech Universal Primer Mix Long (96% over 26bp)
GCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGA16750.10101466974233721No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG16640.10035128982164128Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)