FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001850061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001850061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731143
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116690.674063321169886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.3546789606635616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44330.2560735883748483No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA27220.15723715487397633No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC25290.1460884513873204No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25250.14585739017516172Clontech SMART CDS Primer II A (96% over 26bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC22920.1323980745669191No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21360.12338668729273086Clontech Universal Primer Mix Long (96% over 25bp)
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA21070.12171149350458049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.11038949410880558No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC18950.10946524926017087No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC18070.1043819025926801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)