FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001850847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001850847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346176
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108650.807101003137777No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89660.6660347532566322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72240.536631168584197No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58520.4347128458685937Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57770.4291415089854521No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49690.3691196396310735No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44150.32796603118760104Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA38870.2887438195302843Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37690.2799782495008082Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37620.27945825805838165Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36950.27448119710944185Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG36530.2713612484548826Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.26541848911286486No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.1981910240562898No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25590.19009401445279073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG19020.14128910335647046Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC18490.13735202529238374Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18270.1357177664733289Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA17210.12784361034515546Clontech Universal Primer Mix Long (96% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG17100.12702648093562802No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16670.12383224778929353Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16600.12331225634686699No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.11335813444898735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)