FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001850951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001850951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540804
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT149050.9673521096778046No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC143100.9287359067084457No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA84130.5460136396322959No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT82990.5386149049457296No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA81640.5298532454484801No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC80920.5251803603832804No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG79860.5183008351484031No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC69080.44833736153332937No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT65270.42361001139664745No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC64200.416665584980309No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC63680.4132907235443314No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT62980.4087476408420539No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT62410.4050482734987708No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC56580.36721088470694524No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA56500.3666916752552563No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT55420.35968234765745677No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC53320.34605309955062424No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA51670.3353444046095415No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC50710.3291138911892752No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA49040.3182753938852703No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT48430.3143164218161427No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG46890.30432163987113225No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA46220.299973260713238No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA41520.2694697054265176No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG41040.2663544487163844No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG40830.2649915239057012No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT40200.26090274947365144No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG39810.2583716033966682No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC39060.2535040147870852No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC37810.24539136710444676No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT37110.24084828440216924No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC37080.24065358085778593No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG36780.2387065454139527No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG36700.23818733596226385No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC36410.23630520169989175No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG35880.23286543908245305No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT35490.23033429300546984No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC35430.22994488591670323No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC35380.2296203800093977No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG33830.21956069688292604No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC31530.20463342514687138No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA30800.19989563890021053No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT30260.19639097510131073No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC29820.193535323117022No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG29500.1914584853102666No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT28650.18594188488607247No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT28170.1828266281759393No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA27970.18152860454671715No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA27200.17653121357421192No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA26610.17270204386800658No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT26510.1720530320533955No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG26200.17004109542810117No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA26090.169327182432029No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA25980.1686132694359568No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC25380.16471919854829037No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT24770.16076022647916283No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG24150.15673635322857418No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT24070.1562171437768853No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG23820.15459461424035761No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG23790.1543999106959743No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG23150.15024623508246346No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG22750.14765018782401915No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG22670.1471309783723303No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT22590.14661176892064143No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT21920.14226338976274724No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA21370.13869382478238634No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA21160.13733089997170309No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT20690.13428054444303103No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT20320.13187920072897008No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT20140.13071097946267013No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT19900.12915335110760356No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC19750.12817983338568695No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT19630.12740101920815367No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT19460.12629769912331484No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA19300.12525928021993712No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG19290.12519437903847602No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACC18780.12188441878395954No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC18650.12104070342496515No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGT17720.11500489354908217No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA17620.1143558817344711No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC17490.1135121663754767No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT17110.11104592147995462No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG17050.11065651439118797No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG17000.11033200848388244No Hit
AAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG16910.10974789785073247No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG16730.10857967658443254No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG16540.10734655413667152No Hit
GAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG16520.10721675177374929No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT16360.10617833287037157No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG15660.10163525016809406No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT15540.10085643599056077No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG15530.10079153480909966No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)