FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001851295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001851295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615930
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA694872.6563019652666546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA625102.389589935510507Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT550112.1029232433589584Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC549212.099482784325269Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT452331.7291364830098663Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG324261.2395591625158167Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA303551.160390377418356Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG291721.1151674547866341Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG196650.7517402988612081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG188950.7223052604618625Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC179540.6863333498985065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC174900.668595872213706Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC161270.6164920315146047Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC158390.6054825626067976Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG131310.501962973015333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT124760.476924076714591Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA121440.4642326056125355Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA112230.4290252415011105Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC109130.4171747714961792Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG94480.3611717438922295Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC94210.36013960618212265Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC94210.36013960618212265Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG86850.33200429675105986Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG74930.28643732821596907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA74190.2836085063438242Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.2801298199875379No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG72880.2786007270836758Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70960.2712610811451377Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC68880.2633097980450547Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA67810.2592194745272236Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA66500.2542116952670752Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC64230.24553409303765772Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60090.22970798148268493Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA57610.22022760547873985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA56170.21472287102483628Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC55720.21300264150799142Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT55230.21112950270076036Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53720.2053571769886809Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG53300.20375162943962566Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC52510.20073167095449804Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT52240.1996995332443911Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC51380.19641198350108757Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC51300.19610616492031513Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT51230.19583857366213928Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG51060.19518870917799788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA47580.18188560091439754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46050.176036820557125No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG45540.17408722710470081Clontech Universal Primer Mix Long (96% over 26bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC45330.1732844533301732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA42320.16177802922861087Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG41930.1602871636473453Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG39850.15233588054726235Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG38670.14782505648086913Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT38350.14660178215777944Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT38190.1459901449962346Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA37900.14488155264093458Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG37430.14308486847889662No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA37020.14151754825243795No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG36590.1398737733807862No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC36450.13933859086443445No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA36330.13887986299327582Clontech Universal Primer Mix Long (96% over 26bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT35130.1342925842816895No Hit
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA34920.13348981050716188Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34450.1316931263451239No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT34170.13062276131242045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT31760.12140997656665126No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC31590.12076011208250986Clontech SMART CDS Primer II A (96% over 26bp)
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31230.11938392846903396No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30210.11548474156418559Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA29980.11460551314446488No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC29820.11399387598292003No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT29290.11196782788530275No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA29020.11093569017519582No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA28740.10986532514249234Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT28020.10711295791554055Clontech Universal Primer Mix Long (96% over 25bp)
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT27180.10390186281743012No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26960.10306086172030596No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA26940.10298440707511287Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG26560.10153176881644385Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG26560.10153176881644385No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG26350.10072899504191626No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)