FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001851983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001851983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762806
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG30900.4050833370476897No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG30840.40429676746118937No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA26850.35198988995891484No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23940.313841265013647No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23570.3089907525635614No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT23490.30794199311489423No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC23340.3059755691486433No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC22540.2954879746619717No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA22490.29483250000655475No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC22100.28971979769430234No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT20970.2749060704818787No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG20350.26677818475470827No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20040.262714241891123No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT19510.25576621054370313No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC19190.25157117274903446No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18440.2417390529177799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.2416079579866965No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT17710.23216912294869207No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17680.23177583815544187No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG16590.21748649066735187No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC16570.21722430080518507No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA16200.21237378835509946No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG15970.20935860494018138No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA15900.20844094042259761No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG15830.20752327590501385No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA15830.20752327590501385No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA15570.2041148076968456No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA15320.20083743441976074No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG15220.19952648510892676No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT15140.1984777256602596No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG15120.19821553579809284No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC14980.1963802067629253No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG14880.19506925745209136No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG14480.18982546020875557No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG14470.18969436527767217No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA14300.18746575144925445No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA14010.183663998447836No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT13770.18051772010183453No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT13730.17999334037750095No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG13560.17776472654908324No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG13470.17658487216933272No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG13430.1760604924449991No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG13230.1734385938233312No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG13140.17225873944358067No Hit
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT13050.17107888506383012No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA13010.17055450533949654No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG12680.1662283726137445No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG12680.1662283726137445No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12670.1660972776826611No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG12560.1646552334407438No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT12360.1620333348190759No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC12100.15862486661090763No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG11830.15508530347165597No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA11600.1520701200567379No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG11440.14997260115940356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.14997260115940356No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11050.14485989884715117No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG11030.1445977089849844No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC10940.14341785460523382No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC10850.14223800022548327No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10790.1414514306389829No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10750.14092705091464935No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT10700.14027157625923237No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG10660.13974719653489878No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC10370.13594544353348034No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC10270.1346344942226464No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG10240.1342412094293962No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG10230.1341101144983128No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC10200.13371682970506263No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10160.13319244998072904No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCG10000.13109493108339473No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA9740.12768646287522645No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC9730.12755536794414307No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC9720.12742427301305967No Hit
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA9650.12650660849547593No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCG9580.12558894397789214No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAG9570.12545784904680876No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC9470.12414689973597481No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC9450.12388470987380802No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA9350.12257376056297407No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9300.1219182859075571No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC9300.1219182859075571No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG9290.1217871909764737No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT9200.12060733659672315No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG9180.12034514673455636No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG9140.11982076701022278No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA9070.11890310249263902No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9010.11811653290613865No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG8960.11746105825072167No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT8840.11588791907772093No Hit
GTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGG8760.11483915962905379No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG8760.11483915962905379No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC8760.11483915962905379No Hit
CATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCA8590.11261054580063608No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA8460.11090631169655195No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG8420.11038193197221836No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT8250.10815331814380065No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA8180.10723565362621688No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8140.10671127390188331No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC8080.10592470431538294No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT8070.10579360938429955No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC8060.10566251445321614No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC7950.10422047021129881No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG7940.10408937528021542No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7910.10369609048696522No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC7800.10225404624504789No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG7790.1021229513139645No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT7750.10159857158963091No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA7720.10120528679638072No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA7640.10015652734771358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)