FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001852022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001852022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822743
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA276290.9787996994412882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT198770.7041732102426612Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA156190.5533270297721047Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC149470.5295203991294992Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT132260.46855133464151716Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG113980.40379163104823923Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG99720.3532733940000914Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG96690.34253915429070236Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG70870.251067844291882Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC69970.24787945625939023Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA69020.24451393555842668Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT63890.2263401237732234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC59640.21128384695312327Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50530.17901027475756737Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC48530.1719249680186967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG36350.12877544997897436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.1265435783562301No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG35420.1254807823453995Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA35170.12459511900304067Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA34580.12250495351507382Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC34520.12229239431290768Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC33740.11952912468474813Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG33110.11729725306200388Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC31630.11205412607523958Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC30220.10705898482433575Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT29930.1060316153471995Clontech Universal Primer Mix Long (100% over 23bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT29580.10479168666789715Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA28930.10248896197776416Clontech SMART CDS Primer II A (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC28820.1020992701071263Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)