FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001852434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001852434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950807
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT252520.8557658972613255Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA244420.8283157793783194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT119360.40449951487847224Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG110730.3752532781710224Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG87730.297308498997054Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC87590.2968340525151255Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG75320.25525220727753456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC69610.23590156862173636Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG57090.1934724975235588Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT53000.17961188244436183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA44260.14999286635825385Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT42300.1433506156112548Clontech Universal Primer Mix Long (96% over 27bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41080.13921615341159216Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG39980.13548835962501105Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.12335608530141076No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA35470.12020440510002856No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC34920.11834050820673803Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA33910.1149177157299681Clontech SMART CDS Primer II A (96% over 26bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT33530.11362993242187645No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA33390.11315548593994794Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG33260.11271492849244291Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG32600.11047825222049426Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30540.10349711112926058Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)