FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001852480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001852480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549457
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47300.30526823267764125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44020.2840995264792763Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC41940.2706754688900692No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT29820.19245451793757426No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21820.1408235272098548Clontech Universal Primer Mix Long (96% over 26bp)
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA20110.12978740294180477No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT19110.12333352910083985No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC19100.1232689903624302No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT18160.11720234895192315Clontech Universal Primer Mix Long (96% over 26bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA17480.112813714740067No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA16340.10545629856136698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)