FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001853463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001853463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510586
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA47330.31332211472898597No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT41980.2779053956544017No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG37750.24990301776926307No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG36820.2437464666030269No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT34090.22567400995375303No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC32130.2126989128722231No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA32020.2119707186482597No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG31790.21044813072542706No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA30630.20276899163635836No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG29710.19667863994502796No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA28880.19118408352784946No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA27000.17873858224556563No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26920.1782089864463195No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC26870.1778779890717907No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT26180.17331022530329288No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA26060.17251583160442371No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT25870.1712580415812142No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25080.16602828306365874No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25060.1658958841138472No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC25040.1657634851640357No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG24380.16139431982025518No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT23060.1526559891326942No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC22270.14742623061513874No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG21830.1445134537192851No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA21530.14252746947211214No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG21530.14252746947211214No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC21390.14160067682343144No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21300.14100488154927956No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC21290.1409386820743738No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21020.13915129625191813No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20730.13723151147965093No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20650.13670191568040482No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC20370.1348483303830434No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG20130.13325954298530504No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC19340.1280297844677496No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG18770.124256414398121No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA18630.12332962174944027No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG18380.12167463487679617No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA18370.1216084354018904No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC18280.12101264012773849No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA18210.12054924380339815No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC18030.11935765325509438No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC17930.11869565850603672No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT17890.11843086060641367No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT17510.1159152805599946No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17480.11571668213527729No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC17400.11518708633603118No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT17230.11406169526263317No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC17030.11273770576451787No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT16960.11227430944017752No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG16830.11141371626640258No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA16460.10896433569488927No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT16110.1066473540731875No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16060.10631635669865866No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT16050.1062501572237529No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT15710.10399937507695688No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC15690.10386697612714535No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA15680.10380077665223959No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC15620.10340357980280501No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG15210.10068940133166865No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG15180.10049080290695134No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15150.10029220448223405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)