FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001854560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001854560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503180
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77960.5186338296145505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59710.3972245506193536Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43920.29218057717638607Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32940.21913543288228954Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28480.18946500086483323Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27500.18294548889687198Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG27360.18201413004430608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG26260.1746963104884312Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21720.1444936734123658Clontech SMART CDS Primer II A (96% over 26bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG18800.12506818877313428No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18800.12506818877313428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17820.11854867680517304Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17720.1178834204819117No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG15220.10125201240037786Clontech SMART CDS Primer II A (96% over 26bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15140.10071980734176879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)