FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001854652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001854652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027963
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC89100.8667627142222045No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT69560.6766780516419366No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA53710.522489622680972No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA49560.4821185198299939No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT48750.4742388587916102No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT41460.4033219094461571No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC41110.3999171176394481No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG40970.3985552009167645No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC40450.393496653089654No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT38640.3758890154606732No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT38470.3742352594402717No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC37780.36752295559275966No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA36810.3580868182998804No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC33880.3295838468894309No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC33580.3266654539122517No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA32230.3135326855149456No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA28800.2801657258091974No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG28790.28006844604329145No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC27860.27102142781403615No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC27570.26820031460276295No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA26600.2587641773098837No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA26400.2568185819917643No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC26180.2546784271418329No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG25710.2501062781442523No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG25450.24757700423069703No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC25210.24524228984895372No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG25050.24368581359445815No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC24950.24271301593539846No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC24740.24067014085137306No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG24600.23930822412868946No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG23650.23006664636762217No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG23100.22471625924279376No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT22770.2215060269678967No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT22540.21926859235205937No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT22360.2175175565657519No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC21810.21216716944092345No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT20800.20234191308442034No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT20380.19825616291636955No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20230.19679696642778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA19950.1940731329824128No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC19930.19387857345060086No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA19190.18667987077355896No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT19170.18648531124174703No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18630.18123220388282457Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC18290.17792469184202156No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT17780.17296342378081703No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG17740.17257430471719315No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC17370.1689749533786722No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC17330.1685858343150483No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17040.16576472110377516No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA16500.1605116137448527No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG16230.15788506006539146No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA15560.1513673157496914No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT15550.1512700359837854No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA15360.14942172043157195No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT15340.14922716089976001No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG15160.14747612511345254No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT14880.14475229166808534No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG14740.14339037494540174No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT14130.1374563092251375No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA14100.13716446992741957No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG13830.13453791624795836No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC13810.13434335671614642No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC13760.13385695788661656No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG13690.13317599952527476No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG13660.13288416022755684No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA13440.13074400537762546No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT13420.13054944584581352No Hit
CCGCTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGAT12950.12597729684823286No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG12930.12578273731642092No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA12860.12510177895507912No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT12600.12257250504152387No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT12580.12237794550971193No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12160.11829219534166113No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG11460.11148261172824314No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGG11440.11128805219643119No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT11300.10992613547374759No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG11260.10953701641012371No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC10970.10671590319885055No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT10910.10613222460341472No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA10580.10292199232851766No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG10470.10185191490355197No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG10390.1010736767763042No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT10340.10058727794677436No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACC10290.1001008791172445No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)