FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001854687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001854687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757660
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC85471.1280785576643877No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT69040.9112266715941187No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT52300.690283240503656No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC47500.6269302853522688No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA46880.6187471953118813No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC40170.5301850434231713No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG38360.5062956999181691No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA37830.49930047778687014No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT36490.48161444447377455No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT36010.47527914895863577No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT35470.4681519415041048No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC33710.4449225246152628No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG33500.4421508328273896No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA32380.42736847662539923No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA32120.4239368582213658No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA31660.41786553335269117No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC30090.3971438376052583No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT29030.3831533933426603No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC26900.3550405194942322No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT26590.3509489744740385No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT26010.3432938257265792No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT25960.3426338991104189No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC25800.340522133938706No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG25310.3340548531003352No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG25020.33022727872660557No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG24450.32270411530237836No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG23640.3120133041205818No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA23640.3120133041205818No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC23630.3118813187973497No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC23590.3113533775044215No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA23490.31003352427210096No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA23390.30871367103978037No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC23150.305546023282211No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT22070.2912916083731489No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG21640.2856162394741705No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC21460.28324050365599346No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT21450.2831085183327614No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG21440.28297653300952935No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC21260.28060079719135234No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20580.2716257952115725No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG20350.2685901327772352No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC19620.25895520418129503No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC19160.2528838793126204No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA19110.25222395269646014No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT18870.24905630493889078No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT18830.24852836364596254No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA18640.24602064250455347No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC17610.2324261542116517No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT17520.23123828630256313No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC16870.22265924029247947No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA16190.21368423831269961No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA15480.20431328036322363No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG15080.19903386743394136No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG14920.19692210226222845No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG14780.19507430773697965No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG14580.19243460127233852No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT14570.19230261594910647No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT14230.18781511495921652No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG13940.1839875405854869No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC13730.1812158487976137No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA13710.1809518781511496No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT13290.17540849457540322No Hit
CCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT13240.17474856795924293No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG13100.17290077343399413No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT12610.16643349259562337No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT12570.16590555130269513No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT12330.16273790354512577No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG12290.16220996225219755No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA12260.16181400628250137No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG12100.15970224111078848No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT12000.15838238787846792No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC11880.15679856399968323No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT11790.15561069609059472No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC11680.1541588575350421No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT11660.153894886888578No Hit
TTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCT11620.15336694559564976No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG11330.14953937122192013No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC11170.14742760605020722No Hit
GTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACACGGTGGAT11080.1462397381411187No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA10930.14425995829263785No Hit
GAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG10790.14241216376738908No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC10790.14241216376738908No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC10680.14096032521183646No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT10610.14003642794921206No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA10570.13950848665628382No Hit
AAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG10540.13911253068658763No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGG10400.13726473616133886No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC10270.13554892695932214No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG10100.13330517646437715No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA10010.13211730855528867No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA9980.13172135258559248No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCC9930.13106142596943218No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC9870.13026951403003986No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA9520.12565002771691786No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG9420.12433017448459731No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGC9290.12261436528258059No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT9270.12235039463611647No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT9140.12063458543409972No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC8990.11865480558561887No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC8940.1179948789694586No Hit
TTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTC8910.11759892299976243No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC8890.11733495235329833No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG8880.11720296703006626No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGT8780.11588311379774568No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCT8770.11575112847451363No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG8630.11390333394926484No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC8540.11271546604017632No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG8500.11218752474724811No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATA8460.11165958345431988No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC8420.11113164216139165No Hit
TATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACACGGTGGATG8410.1109996568381596No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG8410.1109996568381596No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT8360.1103397302219993No Hit
AATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAT8150.10756803843412611No Hit
GTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAA8120.10717208246442997No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG8100.10690811181796585No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG8020.1058522292321094No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG7930.10466436132302089No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG7890.10413642003009266No Hit
GTACATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCA7890.10413642003009266No Hit
CACACATACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC7870.10387244938362854No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT7830.10334450809070031No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG7800.10294855212100414No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT7750.10228862550484386No Hit
CTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCG7730.10202465485837976No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGG7620.10057281630282712No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA7620.10057281630282712No Hit
GCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTGATTTCTTTTC7580.10004487500989889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)