FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001855069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001855069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189960
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70940.5961544925879861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.42640088742478743No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32770.27538740798010014Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31340.2633701973175569Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.22446132643114056No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20770.17454368214057614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.14723183972570505No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA16820.14134928905173283No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15990.13437426468116576Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15780.1326094994789741Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.13185317153517764No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC14660.12319741840061851No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC12920.10857507815388752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)