FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001856008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001856008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620304
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC24830.40028760091825943No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT24410.39351672728210685No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT22930.3696574582785215No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA22520.36304779591942016No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21240.34241275245685987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21110.3403170058551936Clontech Universal Primer Mix Long (96% over 26bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC20770.33483582243545096No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA18290.29485542572674045No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG17740.2859888054889216No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT17650.2845379039954603No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC15900.2563259305114911No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT14770.23810905620469963No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC13390.21586189997162683No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA12760.20570558951739792No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC12530.20199773014521913No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG12140.19571049034022028No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT12130.19554927906316905No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11160.17991178518919757No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11110.1791057288039413No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA11070.1784608836957363No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC10490.16911062962676365No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT10200.16443550259227735No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG10030.16169491088240606No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC9740.15701978384791973No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9690.15621372746266346Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC9640.15540767107740722No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA9570.15427919213804844No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC9380.15121617787407465No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT9380.15121617787407465No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC9240.1489592199953571No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG9140.1473471072248446No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA9140.1473471072248446No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT8950.1442840929608708No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG8690.14009259975753824No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC8620.13896412081817947No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG8240.13283809229023189No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC8210.13235445845907812No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT8090.1304199231344631No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG8060.12993628930330936No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7770.12526116226882303Clontech Universal Primer Mix Long (96% over 26bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT7690.12397147205241302No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC7600.12252057055895174No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA7490.12074724651138798No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7480.12058603523433671No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7330.11816786607856793Clontech Universal Primer Mix Long (96% over 26bp)
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7260.11703938713920915No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7250.1168781758621579Clontech Universal Primer Mix Long (96% over 26bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA7030.11333152776703036No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6970.11236426010472285No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6940.11188062627356908Clontech SMART CDS Primer II A (96% over 26bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6870.11075214733421031No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG6740.10865640073254405No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA6640.10704428796203151No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG6580.106077020299724No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA6250.10075704815703268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)