FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001856129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001856129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344367
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244130.7299737140092579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161250.48215402197187096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125620.37561667125647397No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA59340.1774326800856485No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52390.15665146797585314Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC47610.14235877820825285Clontech Universal Primer Mix Long (96% over 25bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT47590.14229897615901604No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG45290.1354217404967816No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG44280.13240173701032212No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43990.13153460729638825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.12815579151450782No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT41220.1232520234770885No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA39390.11778013597191934No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA35010.10468348718905551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)