FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001856469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001856469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731804
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56410.32572970151356617No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGA55570.32087926809269407No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG51100.29506803310305324No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG48910.28242226025577954No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC41070.23715154832763982No Hit
GATTAGAGGCATCGGGGGCGCAATGCCCTCGACCTATTCTCAAACTTTAA40960.23651637252252566No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAATTTTAACCCG39640.22889426286115516No Hit
ATCCTAAGAGACGGGGGAAGCCCGTCCGATAGCGCGTTTCGCGCGAGCTT39080.2256606405805738No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCA38800.22404382944028306No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA35630.2057392176019919No Hit
TTCCCAATCCGTCCCCCGACCGGCACGCGGCGACCCGCTCTCGCCGCGCG35070.2025055953214105No Hit
AGGTAGGATGGCGCGGCTGCTTGATTGAGCCGTGCCACGGAATCGAGAGC35060.20244785206640012No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCA32290.18645297042852424No Hit
TCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGATGGT31990.18472067277821277No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT31360.1810828477125587No Hit
GTCCACCGACAGCCGATGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT30890.17836891472707073No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG30620.1768098468417904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30110.17386494083626092No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG29930.17282556224607404No Hit
AGCCAGGGCGGAGCGGCCGTTGGTGCAGATCTTGGTGGTAGTAGCAAATA29460.17011162926058607No Hit
ATCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCATTGCGCCCCCGA29330.16936096694545108No Hit
GAGTAGGAGGGCGCAGCGGTCGCTGTAAAACCTGTGGCGTGAGCCAGGGC29310.16924548043543033No Hit
TTCCACATCCGACCGGGGCGCATCGCCGGCCCCCATCCGCTTCCCTCCCG29250.16889902090536804No Hit
GTCTTAATCGACCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTGG28030.1618543437941014No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT27140.15671519409817738No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC25490.14718755702146433No Hit
AACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG25170.1453397728611321No Hit
TTTCCAGGGTGGGCAGGTTGTTAAACAGAAAAGATAACTCTTCCCGAGGC25050.1446468538010075No Hit
GTACATGGGCAGAAATCACATTGCGTTAACATCCGCAAGGACCATCGCAA24780.14308778591572718No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT24590.14199066407052993No Hit
GTCGGTGGACTGCTCGAGCTGCTCGCGCGGCGAGAGCGGGTCGCCGCGTG24320.1404315961852496No Hit
ACCTGATAGAACTCGCATATGAGCTCCAGCTATCCTGAGGGAAACTTCGG24250.14002739340017692No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC24060.13893027155497964No Hit
CCTCTAATCATTGGCTTTACCTGATAGAACTCGCATATGAGCTCCAGCTA23910.1380641227298239No Hit
GTCCCGAACCCATCGGCTGTCGGTGGACTGCTCGAGCTGCTCGCGCGGCG23810.13748669017972012No Hit
CCCCTTGTCCGTACCAGTTCTGAGCTGGCTGTTCGACGCCCGGGGAAGGC23500.13569664927439826No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT22990.13275174326886877No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC22810.1317123646786819No Hit
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCG22660.13084621585352615No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAA22320.12888294518317314No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA22080.12749710706292397No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAA21900.1264577284727371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.1245522010573945No Hit
GAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAAGGGCC21140.12206924109194806No Hit
TACCAGTTCTGAGCTGGCTGTTCGACGCCCGGGGAAGGCCCCCGAAGAGG20960.12102986250176118No Hit
ATCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC20650.11923982159643932No Hit
TCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTC20300.11721880767107595No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT20010.11554425327577485No Hit
GTTATGAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAA19890.11485133421565026No Hit
GCGTTAACATCCGCAAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT19320.11155996868005848No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT19210.11092479287494429No Hit
GCCCTGGCTCACGCCACAGGTTTTACAGCGACCGCTGCGCCCTCCTACTC19140.1105205900898716No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGAT18970.10953895475469512No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGTTGGAAGAGCACC18860.10890377894958089No Hit
TGATTGAGCCGTGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGT18690.1079221436144044No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGGGCC18590.10734471106430057No Hit
TCGGTGGACTGCTCGAGCTGCTCGCGCGGCGAGAGCGGGTCGCCGCGTGC18520.1069405082792279No Hit
GTTCTGAGCTGGCTGTTCGACGCCCGGGGAAGGCCCCCGAAGAGGCCGTT18460.1065940487491656No Hit
ATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGAAGCG17900.1033604264685842No Hit
GGTTAGTCGATCCTAAGAGACGGGGGAAGCCCGTCCGATAGCGCGTTTCG17810.10284073717349076No Hit
GGATAGCTGGAGCTCATATGCGAGTTCTATCAGGTAAAGCCAATGATTAG17630.10180135858330389No Hit
GTTTAACAACCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCA17360.10024229069802357No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAA17350.10018454744301318No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)