FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001856470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001856470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731804
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGA49900.2881388425018074No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG46080.2660809190878413No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG45950.2653302567727064No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAATTTTAACCCG42940.24794953701458133No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC39410.22756616799591642No Hit
GATTAGAGGCATCGGGGGCGCAATGCCCTCGACCTATTCTCAAACTTTAA35130.20285205485147279No Hit
ATCCTAAGAGACGGGGGAAGCCCGTCCGATAGCGCGTTTCGCGCGAGCTT34980.20198590602631705No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCA34760.20071555441608865No Hit
TTCCCAATCCGTCCCCCGACCGGCACGCGGCGACCCGCTCTCGCCGCGCG34650.20008037861097444No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA33920.19586512099521655No Hit
AGGTAGGATGGCGCGGCTGCTTGATTGAGCCGTGCCACGGAATCGAGAGC32720.18893593039397066No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCA32550.18795429505879419No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG32270.18633748391850347No Hit
TCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGATGGT30770.17767599566694614No Hit
TTCCACATCCGACCGGGGCGCATCGCCGGCCCCCATCCGCTTCCCTCCCG30250.17467334640640628No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT29960.17299879201110518No Hit
GTCTTAATCGACCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTGG28730.16589637164482815No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG28510.16462602003459978No Hit
GTCCACCGACAGCCGATGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT27980.1615656275190495No Hit
ATCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCATTGCGCCCCCGA27690.15989107312374842No Hit
TTTCCAGGGTGGGCAGGTTGTTAAACAGAAAAGATAACTCTTCCCGAGGC26780.15463643691780363No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT26450.15273090950246102No Hit
GAGTAGGAGGGCGCAGCGGTCGCTGTAAAACCTGTGGCGTGAGCCAGGGC26350.1521534769523572No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT26020.1502479495370146No Hit
CCTCTAATCATTGGCTTTACCTGATAGAACTCGCATATGAGCTCCAGCTA24860.14354973195581025No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC24790.14314552917073756No Hit
AACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG24710.14268358313065452No Hit
CCCCTTGTCCGTACCAGTTCTGAGCTGGCTGTTCGACGCCCGGGGAAGGC24180.13962319061510425No Hit
GTACATGGGCAGAAATCACATTGCGTTAACATCCGCAAGGACCATCGCAA23720.13696700088462668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.1354656762543567No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT23360.13488824370425292No Hit
ACCTGATAGAACTCGCATATGAGCTCCAGCTATCCTGAGGGAAACTTCGG23190.1339066083690764No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC22420.12946037773327698No Hit
GTCGGTGGACTGCTCGAGCTGCTCGCGCGGCGAGAGCGGGTCGCCGCGTG21990.12697741776783053No Hit
GTCCCGAACCCATCGGCTGTCGGTGGACTGCTCGAGCTGCTCGCGCGGCG21670.1251296336074983No Hit
GAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAAGGGCC21530.12432122803735296No Hit
TACCAGTTCTGAGCTGGCTGTTCGACGCCCGGGGAAGGCCCCCGAAGAGG21220.12253118713203111No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT20780.1199904839115743No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAA20700.11952853787149122No Hit
ATCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC20620.11906659183140818No Hit
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCG20580.11883561881136663No Hit
TCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTC20550.11866238904633548No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA20510.11843141602629398No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAA20270.1170455779060448No Hit
GCGTTAACATCCGCAAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT19950.11519779374571255No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC19440.11225288774018306No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAA18890.10907700871461204No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT18340.10590112968904102No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGTTGGAAGAGCACC18150.10480400784384376No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGAT18130.10468852133382299No Hit
GCCCTGGCTCACGCCACAGGTTTTACAGCGACCGCTGCGCCCTCCTACTC17980.10382237250866726No Hit
GTTATGAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAA17780.10266750740845962No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTGGTTCCCTCC17710.10226330462338694No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)