FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001857235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001857235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209898
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT176551.4592139172062437No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC112720.9316487836164702No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA96890.8008113080606795No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT81740.6755941409937036No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT76150.6293918991518294No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC64550.5335160484602834No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC63750.5269039208263837No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA60780.502356396985531No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC58500.4835118332289168No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT58250.4814455433433232No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG55360.4575592322658605No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA52950.43764019776873747No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT49930.4126794159507661No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA49540.40945600372923996No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC47150.38970227242296457No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC45500.37606475917804644No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG45340.37474233365126647No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC44550.3682128576127905No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA43900.36284050391024697No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT43780.361848684765162No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT43530.35978239487956837No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA40690.3363093417792244No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC40590.3354828258249869No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC40270.332837974771427No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA39210.32407690565650993No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG38280.3163903072821015No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG38280.3163903072821015No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT37330.30853840571684554No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT35340.29209073822752No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC34770.28737959728836643No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC34340.28382557868514535No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT33700.2785358765780256No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG33350.27564307073819444No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG31560.2608484351573438No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT31290.2586168420809027No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA30850.2549801718822578No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG30040.2482853926529344No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC29020.23985492991971222No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG28870.23861515598835606No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG28140.23258158952242255No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC27660.22861431294208273No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG27020.22332461083496297No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC26340.21770430234614818No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC25980.2147288449108933No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG25140.20778611089529864No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT24170.19976890613919523No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA24080.1990250417803815No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC24050.19877708699411026No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT24050.19877708699411026No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC23850.19712405508563532No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC23680.1957189779634316No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA23110.1910078370242781No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT22540.18629669608512453No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT22410.18522222534461583No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG22280.18414775460410712No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT22260.18398245141325964No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG22170.18323858705444593No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT21400.17687441420681743No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA21170.17497342751207126No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG21100.17439486634410503No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC20960.17323774400817257No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT20510.169518422214104No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA20340.16811334509190032No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT19650.1624103850076618No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG19450.16075735309918687No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT19370.16009614033579692No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA19180.1585257600227457No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT19110.15794719885477948No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC19020.15720333449596577No Hit
TTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCT18850.15579825737376207No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT18820.15555030258749086No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT18280.15108711643460854No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA18040.1491034781444386No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG17330.14323521486935262No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCT17160.14183013774714892No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG16750.13844142233477533No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA16720.1381934675485041No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT16510.13645778404460543No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA16250.13430884256358802No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT16160.13356497820477428No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG15680.12959770162443446No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG15510.12819262450223076No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG15470.1278620181205358No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT15120.12496921228070466No Hit
GAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG15070.12455595430358592No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG14900.12315087718138223No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGT14260.11786117507426246No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACC14180.1171999623108725No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG13990.1156295819978213No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC13780.11389389849392262No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG13690.1131500341351089No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG13680.11306738253968515No Hit
TTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGG13510.11166230541748148No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGG13390.11067048627239652No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC13310.11000927350900654No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT13250.10951336393646408No Hit
ACCCTGTATCGCACGCCTTTCCAGACGCTTCCACTAACACACACACTGAT13050.10786033202798914No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG12870.10637260331036169No Hit
AAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG12760.10546343576070048No Hit
GGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGA12760.10546343576070048No Hit
CCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT12640.10447161661561552No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT12640.10447161661561552No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC12520.10347979747053057No Hit
ACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCT12460.1029838878979881No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATA12430.10273593311171685No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC12410.10257062992086935No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGC12310.10174411396663188No Hit
GATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT12160.1005043400352757No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACC12140.10033903684442821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)