FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001857298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001857298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074873
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC85550.7959079816871389No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT73360.6824992347933199No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA63670.5923490496086514No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT61070.5681601454311347No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC54880.5105719466392774No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT53410.49689591235429675No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA50440.46926474104382565No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48150.4479598985182435No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT42650.3967910627581119No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG41710.38804584355547117No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC38520.35836791881459484No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT38490.35808881607408505No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC36440.3390167954725814No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG34220.3183631926748555No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA33320.30999011045956126No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC31720.2951046309657048No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG29830.2775211583135868No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC29670.2760326103642012No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT29020.269985384319822No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA28980.26961324733247555No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG27350.25444866509810926No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC27240.2534252883829066No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG27080.251936740433521No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA27080.251936740433521No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA27060.2517506719398478No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC27030.25147156919933794No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG26730.2486805417942399No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC26040.24226117876251424No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA25740.2394701513574162No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC25170.23416719928772983No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC25040.23295775407885397No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC25040.23295775407885397No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG24280.22588715131927214No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC24220.2253289458382525No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG23710.22058419924958578No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT23350.21723496636346806No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT22370.20811761017348096No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC22040.20504748002787304No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT22000.20467534304052667No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA21700.20188431563542855No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA20100.18699883614157206No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG20060.18662669915422567No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT19970.18578939093269622No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT19650.18281229503392493No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA19520.1816028498250491No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA19180.1784396854326046No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG18220.1695083977362907No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT18200.1693223292426175No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT18010.16755467855272205No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT17960.16708950731853903No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT17640.16411241141976773No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC17230.16029800729946703No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT16670.15508808947661723No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA16580.15425078125508782No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT16560.15406471276141462No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT16370.15229706207151916No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA16230.1509945826158067No Hit
CCGCTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGAT16190.1506224456284603No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG16090.14969210316009426No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT16060.14941300041958447No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC15790.14690107575499617No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT15520.1443891510904079No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG15190.14131902094479998No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG14960.13917923326755813No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG14890.1385279935397019No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG14490.13480662366623777No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC14250.13257380174215933No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG14100.13117828803961026No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG13870.12903850036236839No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA13350.12420071952686502No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13340.12410768528002844No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG13210.1228982400711526No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT13170.12252610308380618No Hit
TTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCT12870.1197350756787081No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC12770.11880473321034205No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG12680.11796742498881264No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA12640.11759528800146621No Hit
AAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG12400.11536246607738775No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT11870.11043165099504779No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT11810.10987344551402817No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG11700.10885006879882554No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACC11650.10838489756464253No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGG11640.10829186331780592No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT11530.10726848660260327No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC11450.10652421262791047No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA11310.10522173317219802No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC11190.1041053222101588No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG11130.10354711672913917No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT11080.10308194549495614No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA10980.10215160302659013No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGC10930.1016864317924071No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC10910.1015003632987339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)